Question

2. You are given a mixed broth culture of Pseudomonas fluorescence (Gram-negative) and Clostridium sporogenes (Gram-positive). You are instructed to isolate and purify each bacterial species. How are you going to do it? Your goal is to have a plate of pure P. fluorescence with isolated colonies and a plate of pure C. sporogenes with isolated colonies. Explain in detail how you are going to perform the exercise. You can use any media you like such as nutrient agar plate, trypticase soy broth (TSB), MSA, EMB, MacConkey agar, etc. You can also use any lab equipment that we have used in the lab. Pay attention to the incubation temperature, incubation time and oxygen requirement of the organisms that you are culturing. When trying to explain to me how you are going to perform the exercise, imagine that you are giving instructions to your son or daughter.