Question

Question 2 5 pts Transfer 0.1 mL of the bacterial suspension to the tube marked A1 (containing 0.9 mL of 0.1M MgSO4). Transfer 0.01 mL (10 PL) of the bacterial suspension from A1 to the tube marked A2 (containing 0.99 mL of 0.1M MgSO4). Transfer 0.01 mL of the bacterial suspension from A2 into the microcentrifuge tube marked A3 (containing 0.99 mL of 0.1M MgSO4). Close the lid, and mix. What is the dilution factor? A1 - 10x (1:10), A2- 1000x (1:1000), A3-100000x (1:100000) A1 - 1x (1:1), A2- 1000x (1:1000), A3-100000x (1:100000) A1 - 10000x (1:10000), A2- 1000x(1:1000), A3-100000x (1:100000) O A1 - 100x (1:100), A2- 1000x(1:1000), A3-100000x (1:100000)

Answer 1

A1 = 1:10, A2 = 1:1000, A3 = 1:100000.

When you add 0.1 ml of bacterial culture to 0.9 ml of diluting solutions, the dilution factor of A1 become 1:10.

By the same way, the dilution factor of A2 become, (1/10) * (1/100) = 1/1000.

At last, the dilution factor of A3 becomes, (1/1000) * (1/100) = 1/100000.