You are interested in studying the effects of two newly developed cancer drugs on the expression of a well-characterized oncogene, Ras. To do this, you go back to your cultured cell system and split melonoma cells into 3 culture dishes and treat one with drug #1, one with drug #2, and one is untreated as your control. You then isolate RNA from these cells and perform qPCR to measure Ras mRNA levels. The amplification plot for drug #1 is labeled "A" for drug #2 is labeled "C", and the untreated control is labeled "B" in the figure to the right.
a. Estimate the Ct values for each curve on the graph.
b. What are the effects of drug #1 and drug#2 on Ras expression? Explain your reasoning.
Homework Answers
Based on the given data,
a)
The cycle count or CT value of given curves is as follows:
b)
From the obtained qPCR graph, the drug #1 increases the expression of oncogene, Ras, whereas drug#2 decreases the expression of oncogene, Ras. This is because the tube A has more concentration of mRNA than Tube C.
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