Question

23. You want to edit the vinculin gene of a stable human cell line using pCAS-Guide vector. Which of the following you do not have to have to do?
A. Clone part of the vinculin gene in the pCAS-Guide vector
B. Transfect the recombinant pCAS-Guide vector to the cell line
C. Inject a small amount of cas 9 protein in the cells
D. Insert a marker gene inside the cell

The siRNA, miRNA and piRNA of eukaryotes

24. An antisense RNA of a gene hybridized with its cognate mRNA. The dsRNA was immediately processed by dicer. The small RNA thus produced are most certainly _______ .
A. siRNA
B. miRNA
C. piRNA
D. S6 RNA

Answer 1

23. A. If you want to edit the vinculin gene of a stable human cell line using pCAS-Guide vector you should not insert the marker gene in to the cell 24. A. An antisense RNA of a gene hybridized with its cognate mRNA. The dsRNA was immediately processed by dicer. The small RNA thus produced is most certainly siRNA (short interfering RNA) During a basic RNAi response, dsRNA is cleaved by the endonuclease Dicer into short interfering RNA (siRNA), of which one strand is then loaded onto an Argonaute protein