Question

There are amino acid residue at the following positions:

Arginine - 136

Valine - 58

Glycine - 235

Glutamate - 97

Which of the following amino acid substitutions would be least likely to affect the activity of this enzyme? Why?

A. Tryptophan at position 235

B. Aspartate at position 58

C. Lysine at position 136

D. Asparagine at position 97

Answer 1

Bructure of amino acids are: CH2 と C CH₂ valine (v val NH Arginine & Han – 4 – 4 Go bycine (6) Gly - Hot 1 - HAN KLA - - - - - N1 Glutamate CE Сол Tryptophan (w) Tip u-u- ch ch ch とれ ch "C=0 HN eragine (W) Asn Lysine (K) Lys Here, Arg frys both are basic amino acid. Ghu is acidic amino acid val, tre, Gly are non polar amino acids. Aswin polar amino acid,

# At 235 glycine in present. Glycine & Tryp to phan both are non polar amino acid. But tryptophanis aromatic amino acid & glycine is non- - aromatic amino acid So, at position 235 trh subtitution is not recomended & it will affect the activity # At position so, val is present. val is not polar & Ang in acidic. amino acid. So, this will affect the activity much more. #atposition 136, Arg in present. Ang in basic amino acid & on the other hand lys is a basic amino acid. So, this change acill make reast likely to affect the activity of the enzyme # am in polar & Glutamate in ridic in nature. So this charage will make a large affect on enzyme octivity Answer: (e) MO