(Q1-6) The picture shown below illustrates how we can genotype sickle cell anemia with molecular biology...
(Q1-6) The picture shown below illustrates how we can genotype sickle cell anemia with molecular biology techniques. Given the information we learned through the lectures, answer the following questions. (a) Normal allele (A) Pro Glu Glu (translated sequence) Left primer CCTGAGGAGI GGACTCCTC Right primer Mstil restriction site 300 400 500 bp Left primer Pro Val Glu CCTGTGGAGI GGACACCTC No restriction site Right primer Sickle-cell allele (S) (b) AS SS 500 bp (Sallele) -300 bp (A allele) 200 bp 3. In the pedigree (b), what is the genotype of the daughter? A. A/S B. S/A C. AJA D. S/S E. none of the above 4. Which statement is incorrect about the sickle cell anemia of this family? A. transition mutation B. missense mutation C. gain-of-function mutation D. dominant trait E. pleiotropbx