Question

1. Describe the two yeast expression plasmids used to transform a strain of yeast.

2. How do the plasmid vectors (how do their expression products) lead to us knowing whether a known protein and unknown protein bind or interact?

Questions on DNA Testing for Genetic Diseases Associated with Mutations:

3. What are the factors that lead to limits of detecting genetic diseases with DNA molecular testing?

4. What is the difference between information gathered by screening for a disease and by a DNA molecular test for a disease

5. Explain how restriction fragment length polymorphism can reveal a mutation associated with a disease.

Answer 1

Answer-1 the plasmid used in yeast are called shuttle vector. Examples are as follows- yeast episomal plasmid and pESC vector.

Answer 2 The plasmid lead us to know the binding and interaction of two proteins by activating or inhibiting the expression of specific function in the target cells.

Answer-3 The factors associated with the limits of DNA test for the disease include the little knowledge of the biomarker, contamination of the foreign substance in the sample, absence of proper facilities for DNA processing etc.

Answer-4 The screening information reveal about the class characteristics of a disease while the DNA molecular test describe the individual Characteristics of a disease with the help of specific biomarker which originate in that specific disease.

Answer-5 RFLP separate the DNA after Cutting it from some specific site. In mutated sequence the restriction enzyme will not work due to alteration of the sequence and the band size will show the different position as compared to normal sequence. This will make clear the mutation associated with the disease.