The conversion of G6P to fructose-6-phosphate (F6P), catalysed by phosphohexose isomerase (PI), is thermodynamically unfavourable under...
Question 1 - Linked Reactions (10 marks) The reaction catalysed by pyruvate kinase is: Phosphoenolpyruvate+ ADPpyruvateATP Keg 3.63 x 105 a) Calculate the AG" for this reaction. Show your working. 3 marks b) The hydrolysis of ATP has following equation: AG 30.5 kJ/mol Calculate the AG" for the following reaction: Phosphoenolpyruvate pyruvateP Show your working. 2 marks c) At 37 °C, the steady-state concentrations of phosphoenolpyruvate, ATP, and ADP have been measured to be 23 HM, 1.85 mM and 140...
Glucose-6-P isomerase catalyses the following glycolytic reaction: Glucose-6-P (G6P) fructose-6-P G=1700 kJ/mol (Change in free energy) If 7.2 g of G6P* is dissolved in 980 mL of buffer and 150 µL of this is incubated with enzyme at 25°C & 37°C, what would be the equilibrium concentration of F6P at these two temperatures? [15 marks] *- The periodic table may be used to determine the molecular weight of G6P