Glucose-6-P isomerase catalyses the following glycolytic reaction: Glucose-6-P (G6P) fructose-6-P...
Glucose-6-P isomerase catalyses the following glycolytic reaction:
Glucose-6-P (G6P) fructose-6-P G=1700 kJ/mol (Change in free energy)
If 7.2 g of G6P* is dissolved in 980 mL of buffer and 150 µL of this is incubated with enzyme at 25°C & 37°C, what would be the equilibrium concentration of F6P at these two temperatures? [15 marks] *- The periodic table may be used to determine the molecular weight of G6P
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Glucose-6-P isomerase catalyses the following glycolytic reaction: Glucose-6-P (G6P) fructose-6-P...
The conversion of G6P to fructose-6-phosphate (F6P), catalysed by phosphohexose isomerase (PI), is thermodynamically unfavourable under...
The conversion of G6P to fructose-6-phosphate (F6P), catalysed by phosphohexose isomerase (PI), is thermodynamically unfavourable under standard conditions. AG 1.7 k/mol a) Using the data below calculate the mass action ratio (Q) and the AG at 37°C for each pair of G6P and F6P concentrations. [G6P] (HM) [F6P] (HM) Q AG at 37°O 0.8 3.3 2.1 1.2 2.6 0.1 1.3 2.2 2 marks b) Plot your calculated AG values against the natural log (In) of Q. Remember to clearly label...
9. One of the important reactions in the biochemical pathway glycolysis is the reaction of glucose-6-phosphate(G6P)...
9. One of the important reactions in the biochemical pathway glycolysis is the reaction of glucose-6-phosphate(G6P) to form fructose-6-phosphate (F6P): a) Is the reaction spontaneous or nonspontaneous under standard state conditions? b) Standard state conditions imply the concentrations of G6P and F6P to be 1 M, however, in a typical cell, they are not even close to these values. Calculate ΔG when the concentrations of G6P and F6P are 120 μM and 28 uM respectively, and discuss the spontaneity of...
14. The second step of glycolysis is the following reaction: Glucose-6-phosphate = Fructose-6-phosphate At pH 7...
14. The second step of glycolysis is the following reaction: Glucose-6-phosphate = Fructose-6-phosphate At pH 7 and 25 °C, IM of G6P and F6P will equilibrate to [F6P]/[G6P] = 2.43. R, the molar gas constant, is 8.3145 Jmol'K'. A) What is the standard free energy of this reaction at pH 7 and 25 °C? (2 points) B) In the cell the overall AG is -1400 Jmol"!. Calculate the ratio of products to reactants. (2 points)
Question 1 - Linked Reactions (10 marks) The reaction catalysed by pyruvate kinase is: Phosphoenolpyruvate+ ADPpyruvateATP Keg 3.63 x 105 a) Calculate the AG" for this reaction. Show your work...
Question 1 - Linked Reactions (10 marks) The reaction catalysed by pyruvate kinase is: Phosphoenolpyruvate+ ADPpyruvateATP Keg 3.63 x 105 a) Calculate the AG" for this reaction. Show your working. 3 marks b) The hydrolysis of ATP has following equation: AG 30.5 kJ/mol Calculate the AG" for the following reaction: Phosphoenolpyruvate pyruvateP Show your working. 2 marks c) At 37 °C, the steady-state concentrations of phosphoenolpyruvate, ATP, and ADP have been measured to be 23 HM, 1.85 mM and 140...
The conversion of G6P to fructose-6-phosphate (F6P), catalysed by phosphohexose isomerase (PI), is thermodynamically unfavourable under standard conditions. AG 1.7 k/mol a) Using the data below calculate the mass action ratio (Q) and the AG at 37°C for each pair of G6P and F6P concentrations. [G6P] (HM) [F6P] (HM) Q AG at 37°O 0.8 3.3 2.1 1.2 2.6 0.1 1.3 2.2 2 marks b) Plot your calculated AG values against the natural log (In) of Q. Remember to clearly label...
9. One of the important reactions in the biochemical pathway glycolysis is the reaction of glucose-6-phosphate(G6P) to form fructose-6-phosphate (F6P): a) Is the reaction spontaneous or nonspontaneous under standard state conditions? b) Standard state conditions imply the concentrations of G6P and F6P to be 1 M, however, in a typical cell, they are not even close to these values. Calculate ΔG when the concentrations of G6P and F6P are 120 μM and 28 uM respectively, and discuss the spontaneity of...
14. The second step of glycolysis is the following reaction: Glucose-6-phosphate = Fructose-6-phosphate At pH 7 and 25 °C, IM of G6P and F6P will equilibrate to [F6P]/[G6P] = 2.43. R, the molar gas constant, is 8.3145 Jmol'K'. A) What is the standard free energy of this reaction at pH 7 and 25 °C? (2 points) B) In the cell the overall AG is -1400 Jmol"!. Calculate the ratio of products to reactants. (2 points)
Question 1 - Linked Reactions (10 marks) The reaction catalysed by pyruvate kinase is: Phosphoenolpyruvate+ ADPpyruvateATP Keg 3.63 x 105 a) Calculate the AG" for this reaction. Show your working. 3 marks b) The hydrolysis of ATP has following equation: AG 30.5 kJ/mol Calculate the AG" for the following reaction: Phosphoenolpyruvate pyruvateP Show your working. 2 marks c) At 37 °C, the steady-state concentrations of phosphoenolpyruvate, ATP, and ADP have been measured to be 23 HM, 1.85 mM and 140...
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