What is the molar absorptivity of benzoic acid at 228 nm? (assume path length=1cm) The solution contains .010M HCl. Benzoic acid is 8.74mg/L
given the concentration of benzoic acid = 8.74 mg/L = 8.74 x 10-3 g/L
Molecular mass of benzoic acid = 122.1 g/mol
Hence molar concentration of benzoic acid = (8.74 x 10-3 g /122.1 g/mol) /L = 7.16 x 10-5 M
According to Beer - Lambart law, the absorbance, A can be calculated as
A = ExCxL
where E = molar absorptivity,
C = concentration in mol/L = 7.16 x 10-5 M
L = path length in cm = 1 cm
A = absorbance
=> molar absorptivity, E = A / CL
by putting the value of absorbance, A, C and L we can calculate the value of E.
What is the molar absorptivity of benzoic acid at 228 nm? (assume path length=1cm) The solution...
The plot shows the absorbance spectra for solutions of caffeine, benzoic acid, and Mountain Dew soda, each in 0.010 M HCI. All solutions contain 0,010 M HCI What is the approximate absorbance of benzoic acid at 228 nm? Benzoic acid 8,74 mg/L Absorbance Mountain Dew soda 1:50 dilution Caffeine 10,88 mg/L A What is the molar absorptivity (C) of benzoic acid at 228 nm? Assume that the path length is 1.00 cm. 0.21 0.0- 200 M'cm-1 220 240 260 280...
The molar absorptivity of FD&C Yellow #5 at 430 nm is =2.43*104 M-1cm-1. The absorbance of a sample of Gatorade Lemon-Lime at 430 nm is measured as 0.27 using a 1 cm cuvette. Assuming the path length is 1 cm, calculate the molarity of Yellow #5 in Gatorade.
Assume the molar absorptivity (ε) for CoCl42- at 700 nm is 412.2 M-1 cm-1. If d, the cell path length, is equal to 0.85 cm for the cuvettes being used, and if the measured absorbance (A) at 700 nm is 1.305, what is [CoCl42-]? Express your response to four digits after the decimal.
3. Calculate the molar absorptivity of a 0.200 M compound A if the light path length is 1.0 cm, and the absorbance at 254 nm is 0.428. Show work for credit! 6 pts E=ALIC 4.A 0.15 mol of a solid weak acid, HA, was dissolved in 460 mL of water. a. write an equilibrium reaction for the reaction of HA in water. 2 pts b. Calculate the equilibrium concentrations of each reactant and product if K= 5.7x104.6 pts Show work...
A solution of a specific vitamin has a Imax of 287 nm and a concentration of 4.93 x 10-7M. The absorbance of the solution at 287 nm is A = 0.135. What is the molar absorptivity of the vitamin at 287 nm? A sample path length is 1.00 cm. molar absorptivity: L mol-'cm-1
At 575.0 nm, a solution containing 0.0156 g/L CuSO4 (159.61 g/mol) in a cell of path length 2.00 cm gave an absorbance (A) of 0.1549. Calculate (at 575 nm): a) the molarity of CuSO4 b) the absorptivity of CuSO4 units of (L/g.cm) c) the molar absorptivity of CuSO4 [Report the results, without units, to three sig.fig.]
If the molar absorptivity at a specific wavelength is known for a protein, it is pretty easy to determine its concentration simply by measuring its absorbance and using Beers law. Based on the following information, spectrophotometrically determine the concentration (in mg/mL) of a solution of the protein lysozyme. -the molar absorptivity at 280 nm (converted for units of mg/mL) is & = 0.243 mg.cm -the absorbance of a solution of lysozyme is 0.923 when the path length is 1 cm.
Calculate the expected absorbance of solution of a 5.50x10-6 M solution of Blue # measured at 629 nm in a 1.50cm cell. The molar absorptivity for Blue #1 is 1.30×105 M-1cm-1 at 629 nm 4. A 2.50x10-5 M solution of a specific dye has an absorbance value of 1.300 when measured at 525 nm in a cell with a path length of 2.00 cm. Calculate the molar absorptivity of this dye at this wavelength 5.
A solution contains two components, A and B. At 520 nm component A has a molar absorptivity of 0.50 mmol^-1 and component B has a molar absorptivity of 0.20 mmol^-1. At 610 nm component A has a molar absorptivity of 0.10 mmol^-1 and component B has a molar absorptivity of 0.40 mmol^-1. If the total absorbance at 520 nm is 1.00, and the total absorbance at 610 nm is 0.80, what is the concentration of component A in the solution?
The logarithm (log10) of the molar absorptivity of uric acid is 4.086 in neutral aqueous solution at 293 nm. Calculate the range of uric acid concentrations that can be used in a uric acid assay if the absorbance must be greater than 0.100 and less than 2.000 with a 1.25 cm cell 4.