Question

Question 8 7--22- The following proteins were eluted from a G100 size exclusion column (exclusion limit:100,0000 of protein molecules fractionated from G 100 size exclusion column. The elution curve revealed 5 peaks (below), identify the molecule/s eluted as peak number 2. Myoglobin, MW:68,500, Myosin, MW:524,000, Immunoglobulin M:MW375,000, serum albumin. MW: 68,500, Cytochrome c,MW: 13,370, Polypeptide A: MW: 10,000 and Chymotrypsinogen, MW: 23,240 Question 9

Answer 1

Ans:-peak number 2 is of immunoglobulin MW 375,000.

size exclusion column chromatography separte mixture of protein depending on their moleculr weight and size. protein with heigher molecular weight does not get into the gel hence travel faster through column and elute first and produce curve early than other lower molecular weight molecule. lower molecular weight protein get trpped into gel and travel slower than larger molecule hence elute later than larger molecule. peak 1 is of myosin MW 524,000. peak 2 is of immunoglobulin MW 375,000. peak 3 is of myoglobulin and serum albumin MW 68,500. peak 4 is of chymotrypsinogen MW 23,240. peak 5 is of cytochrome MW 13,370