A solute with a retention time of 4 mins 25 second has a base width of 12 seconds. The column is 30.0m long. How many theoretical plates does the column have? What is length in mm of one theoretical plate?
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A solute with a retention time of 4 mins 25 second has a base width of...
A solute with a retention time of 407s has a width at the base of 13.0s on a column 12.2m long. Find the number of plates and plate heigh. The half width of the same peak is 7.6s. Find the plate height
consider the peaks for trans-chlordane and for cis-chlordane where its retention time is 458.5 seconds and 475.0 seconds respectively. The elution time for unretained solute is 25 seconds. The width base for t-chlordane is 4 seconds and c-chlordane is 4.5 seconds. The open tubular column is 12.5m in length and 0.32 in diameter. Find the adjusted retention time, retention factor, number of theoretical plates and plate height for t-chlordane and c-chlordane peaks. in addition calculate the selectivity factor and resolution...
Two compounds C and D are separated on a column 34 cm long . Given the chromatographic data below calculate The column resolution? , the number of theoretical plates ?the height of theoretical plate and what length of column would be required to give a resolution of 1.5? Compound C retention time 18.3 width at base of peak(min) = 2.18 Compound D retention time 17.7, width at base 1.23
L. Choose the correct answer: 1) Reversed phase liquid chromatography refers to (a) the use of non polar solvent as the eluent in HPLC. (b) the use of polar material as the stationary phase in a chromatographic column. the use of non-polar material as the stationary phase in a chromatographic column 2) A solute with a retention time of 325 seconds has a base width of 15 seconds. The column is 11.5 cm long. The c (2 points) olumn has...
need help with number 2 HW #3 for Chem 318 Due: April 29, 2019 1. Solute A has a K 2.5 for an extraction between water (phase 1) and chloroform (phase 2). If 100 mL of a 0.01 fraction will be extracted? For the same solute and, what fraction will be extracted if 5 extractions wi 80 mL chloroform each are used (instead of one 400 ml extraction)? M solution containing solute A in water is extracted one time with...
A student researcher performed a chromatographic separation of caffeine and aspartame. The retention time for caffeine, is, was found to be 218.0 s with a baseline peak width, wc, of 14.4 s. The retention time for aspartame, 1., was 250.5 s with a baseline peak width, w of 21.1 s. The retention time for the unretained solvent methanol was 40.4 s. Calculate the average plate height, H, in micrometers for this separation, given that it was performed on a 27.7...
0.16 min A chromatogram gives an ideal Gaussian peak with t,9.50 min and wi2 How many theoretical plates does the column have? plates N= What is the plate height of the column in millimeters if the column is 10.1 cm long? mm - 0.16 min A chromatogram gives an ideal Gaussian peak with t,9.50 min and wi2 How many theoretical plates does the column have? plates N= What is the plate height of the column in millimeters if the column...
3. a) b) c) How many theoretical plates produce a chromatography peak eluting at 4.78 min with a full width at half max (FWHM) of 1.20 s? The length of the column is 30.0 m. What is the plate height in microns? What is the peak resolution for this peak and another peak eluted at 4.73 minutes that has a full peak width (measured at the base) of 2.14 seconds? Is this sufficient for quantitative analysis of those two components?...
A chromatogram gives an ideal Gaussian peak with t,= 8.60 min and w 0.17 min. How many theoretical plates does the column have? plates N= What is the plate height of the column in millimeters if the column is 11.7 cm long? H= mm
A chromatogram gives an ideal Gaussian peak with t; = 8.50 min and w1/2 = 0.20 min. How many theoretical plates does the column have? N N=1 = plates What is the plate height of the column in millimeters if the column is 11.1 cm long? H = mm