Question-1:
Orange G and Blue Dextran are the two products in the mixture. It is given that Orange G has smaller molecules as it has low molecular weight. Blue Dextran dye has bigger molecules. This mixture is subjected to the size exclusion chromatography using sephadex polymer gel.
First elution ----------------> bigger sized Blue Dextran ----> because it has higher molecular weight
Final elution ----------------> smaller sized Orange G ------> becuase it has smaller molecular weight
Reason: Pricinple of elution by size exclusion chromatography:
Princple of size exclusion chromatography if “adsorption”. Larger molecules with more molecular weight cannot pass through pores within the stationary phase (polymer sephadex) and thereby they elute first followed by smaller molecules with low molecular weight as these molecules are bounded in the porous particles of polymer stationary phase.
Orange G and Blue dextran are in the mixture. Orange G is a small molecule, whereas...
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