Question

To assist in completing this question, you may reference the Animated Technique Video - Isoelectric Focusing. What characteristics determine the position of a protein on an IPG strip at the end of isoelectric focusing? the protein's three-dimensional structure protein solubility the molecular weight of the protein the pl of the protein local pH in the medium

Answer 1

The characteristics which determine the position of a protein on an IPG strip at the end of isoelectric focusing are as follows:-

4. The pI of the protein

5. Local pH in the medium

As we all know proteins are the biomolecules which is formed by the help of amino acid. Amino acid are those acid which contains both amine group as well as carboxylic group. At Isoelectric point no movement of amino acid takes place.