In this experiment, we are doing quantitative determination of Caffeine by reversed-phase high pressure liquid chromatography...
In this experiment, we are doing quantitative determination of Caffeine by reversed-phase high pressure liquid chromatography (HPLC). Elevated column temperature was employed in this method. What impact does increasing column temperature have on retention? On resolution?
Homework Answers
As column temperature increased peak retantion times are shortened as higher temperature leads to faster exchange of analytes between the mobile phase and stationary phase.
As temperature increased resolution decreased as chances of close peaks may get merge.
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In this experiment, we are doing quantitative determination of
Caffeine by reversed-phase high pressure liquid chromatography...
L. Choose the correct answer: 1) Reversed phase liquid chromatography refers to (a) the use of...
L. Choose the correct answer: 1) Reversed phase liquid chromatography refers to (a) the use of non polar solvent as the eluent in HPLC. (b) the use of polar material as the stationary phase in a chromatographic column. the use of non-polar material as the stationary phase in a chromatographic column 2) A solute with a retention time of 325 seconds has a base width of 15 seconds. The column is 11.5 cm long. The c (2 points) olumn has...
High-pressure liquid chromatography (HPLC) is a method used in chemistry and biochemistry to purify chemical substances....
High-pressure liquid chromatography (HPLC) is a method used in chemistry and biochemistry to purify chemical substances. The pressures used in this procedure range from around 500 kilopascals (500,000 Pa) to about 60,000 kPa (60,000,000 Pa). It is often convenient to know the pressure in torr. If an HPLC procedure is running at a pressure of 4.84×108 Pa , what is its running pressure in torr?
High-pressure liquid chromatography (HPLC) is a method used in chemistry and biochemistry to purify chemical substances....
High-pressure liquid chromatography (HPLC) is a method used in chemistry and biochemistry to purify chemical substances. The pressures used in this procedure range from around 500 kilopascals (500,000 Pa) to about 60,000 kPa (60,000,000 Pa). It is often convenient to know the pressure in torr. If an HPLC procedure is running at a pressure of 5.07×108 Pa , what is its running pressure in torr?
High-pressure liquid chromatography (HPLC) is a method used in chemistry and biochemistry to purify chemical substances....
High-pressure liquid chromatography (HPLC) is a method used in chemistry and biochemistry to purify chemical substances. The pressures used in this procedure range from around 500 kilopascals (500,000 Pa) to about 60,000 kPa (60,000,000 Pa). It is often convenient to know the pressure in torr. If an HPLC procedure is running at a pressure of 2.83x10° Pa , what is its running pressure in torr? Express the pressure numerically in torr. View Available Hint(s) VO AXO o ? 2.83x108 Pa...
Part High-pressure liquid chromatography (HPLC) is a method used in chemistry and biochemistry to purify chemical...
Part High-pressure liquid chromatography (HPLC) is a method used in chemistry and biochemistry to purify chemical substances. The pressures used in this procedure range from around 500 kilopascals (500,000 Pa) to about 60,000 kPa (60,000,000 Pa). It is often convenient to know the pressure in torr. If an HPLC procedure is running at a pressure of 4.59 10 Pa, what is its running pressure in torr? Express the pressure numerically in torr. View Available Hint(s) IVO AL $ VE VEX...
1. Make a table to summarize the liquid chromatography techniques we have learned, including normal phase...
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In my organic chemistry lab we are performing a gas
chromatography experiment and I need help with our prelab
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Question 3 1 pts If compound A elutes (comes off) a GC column earlier than compound B in a temperature gradient GC run. What can be inferred about their respective boiling points? Compound A probably has a lower boiling point than that of compound B. Compound A and B have the same boiling point. Elution time does not correlate...
1.) Describe the goals of a Gel Filtration Chromotography Experiment??? 2.) Explain each key theoretical principle...
1.) Describe the goals of a Gel Filtration Chromotography
Experiment???
2.) Explain each key theoretical principle of a Gel Filtration
Chromotography, and how they help acheive the goal???.
4.) Explain the key equations used in the Gel Filtration
Chromotography experiment and the terms involved in the
equation????
HI im trying to prepare for a lab/report and i have some
questions i could use help with please :) over all having trouble
seeing how everything ties together etc :) thank you...
2. If two different compounds have the same Rr value, how might they be identified using paper chromatography?...
2. If two different compounds have the same Rr value, how might they be identified using paper chromatography? 3. Why is it important to keep the spots on the paper as small as possible? 4. If the solvent pool at the bottom of the beaker touched the spots on the 1.5cm line, what would happen to the spots? How would you address this error? 5. If you were teaching a student how to do paper chromatography, what three pieces of...
5. When all of the sample to be collected has left the column, will the drop...
5. When all of the sample to be collected has left the
column, will the drop of solution coming off the tip of the column
be acidic, basic or neutral?
6. How many H+ ions would be displaced from the ion
exchange column by 1 Cu2+ ion?
7. Binding sites on the ion exchange resin are saturated
with which ion before the sample solution is added?
8. Ions are displaced from the resin binding sites by
any ion that has...
L. Choose the correct answer: 1) Reversed phase liquid chromatography refers to (a) the use of non polar solvent as the eluent in HPLC. (b) the use of polar material as the stationary phase in a chromatographic column. the use of non-polar material as the stationary phase in a chromatographic column 2) A solute with a retention time of 325 seconds has a base width of 15 seconds. The column is 11.5 cm long. The c (2 points) olumn has...
High-pressure liquid chromatography (HPLC) is a method used in chemistry and biochemistry to purify chemical substances. The pressures used in this procedure range from around 500 kilopascals (500,000 Pa) to about 60,000 kPa (60,000,000 Pa). It is often convenient to know the pressure in torr. If an HPLC procedure is running at a pressure of 2.83x10° Pa , what is its running pressure in torr? Express the pressure numerically in torr. View Available Hint(s) VO AXO o ? 2.83x108 Pa...
Part High-pressure liquid chromatography (HPLC) is a method used in chemistry and biochemistry to purify chemical substances. The pressures used in this procedure range from around 500 kilopascals (500,000 Pa) to about 60,000 kPa (60,000,000 Pa). It is often convenient to know the pressure in torr. If an HPLC procedure is running at a pressure of 4.59 10 Pa, what is its running pressure in torr? Express the pressure numerically in torr. View Available Hint(s) IVO AL $ VE VEX...
1. Make a table to summarize the liquid chromatography techniques we have learned, including normal phase LC, reversed-phase LC, HILIC, ion-exchange chromatography, affinity chromatography, and SEC/GPC. It should cover general principle/rationale, advantages, disadvantages, etc. for each of them. 2. If you receive an unknown protein to be analyzed (newly made, just a name offered, no relevant information provided or found from website), what do you plan to do using chromatography? 3. Find a chromatography instrument in campus and do some...
In my organic chemistry lab we are performing a gas
chromatography experiment and I need help with our prelab
assignment, thanks!
Question 3 1 pts If compound A elutes (comes off) a GC column earlier than compound B in a temperature gradient GC run. What can be inferred about their respective boiling points? Compound A probably has a lower boiling point than that of compound B. Compound A and B have the same boiling point. Elution time does not correlate...
1.) Describe the goals of a Gel Filtration Chromotography
Experiment???
2.) Explain each key theoretical principle of a Gel Filtration
Chromotography, and how they help acheive the goal???.
4.) Explain the key equations used in the Gel Filtration
Chromotography experiment and the terms involved in the
equation????
HI im trying to prepare for a lab/report and i have some
questions i could use help with please :) over all having trouble
seeing how everything ties together etc :) thank you...
2. If two different compounds have the same Rr value, how might they be identified using paper chromatography? 3. Why is it important to keep the spots on the paper as small as possible? 4. If the solvent pool at the bottom of the beaker touched the spots on the 1.5cm line, what would happen to the spots? How would you address this error? 5. If you were teaching a student how to do paper chromatography, what three pieces of...
5. When all of the sample to be collected has left the
column, will the drop of solution coming off the tip of the column
be acidic, basic or neutral?
6. How many H+ ions would be displaced from the ion
exchange column by 1 Cu2+ ion?
7. Binding sites on the ion exchange resin are saturated
with which ion before the sample solution is added?
8. Ions are displaced from the resin binding sites by
any ion that has...
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