Question

QUESTION 3 what sampl wil yu okn tundhrxcowr İ. b.Chloroplasts ● d.DCPIP QUESTION 4 o a. The cuvette will have DCPIP, chioroplast solution, and be under aluminum foil. e b. The cuvette will have DCPIP a boiled chioroplast solution, and be 20cm from the light source o c. The cuvette will have DCPIP, chioroplast solution, ascorbic acid, and be 20cm from the ight source. e d. The cuvette will have DCPIP chloroplast solution, and be 20cm from the light source. QUESTION 5 In the experiment in part il, with respect to your light sampie, what do you expect to happen to DCPIP it photosynthesis is occurring? a.DCPIP will become green because of the chiorophyll present e b.DCPIP will become reduced and therefore blue d·There will be no change to DCPIP to save

Answer 1

We are looking the chloroplast sample under the microscope

Question 4.c

Ascorbic Acid is key part during interactions between chloroplasts and mitochondria

To optimise the photosynthesis and to protect against photoinhibition

Question 5.c

In this experiment, a blue dye (2,6-dichlorophenol-indophenol, or DPIP) will be used to replace NADPH in the light reactions. When the dye is oxidized, it is blue. When reduced, however, it turns colorless. Since DPIP replaces NADPH in the light reactions, it will turn from blue to colorless when reduced during photosynthesis. This will allow you to monitor the rate of photosynthesis. In order to allow the DPIP to come into contact with chloroplasts, the cells will need to be carefully disrupted. You will test for photosynthetic activity in blended spinach leaves. The intensity of color, measured as absorbance, will be detected by a Colorimeter.