QUESTION NO.2 [40 Marks] In aqueous solution, adenosine triphosphate (ATP) is hydrolyzed to adenosine diphosphate (ADP)...
QUESTION NO.2 [40 Marks] In aqueous solution, adenosine triphosphate (ATP) is hydrolyzed to adenosine diphosphate (ADP) and inorganic phosphate in the presence of myosin enzyme: myosin АТР -ADP + phosphate Researchers have studied the kinetics of this enzyme-catalyzed and reported the data below for reaction at 25°C in the presence of 0.039g myosin /L. Molecular weight of myosin = 480,000 Substrate concentration Rate (umol/L) (umol/L.) 6.1 0.067 19.2 0.123 30.9 0.148 50.5 0.165 119 0.183 a. Use Lineweaver-Burk. Eadie, and Hanes plots to ascertain that they provide an accurate description of these data. Determine the slopes and intercepts of these plots and the associated values of the Michaelis constant and maximum velocity. Comment on the consistency of the parameter values. [27 MARKS] b. Calculate the following from the Lineweaver—Burk plot 1. Enzyme efficiency and the turnover frequency. [05 MARKS] II. Number of catalytic cycles performed per active site in 2 minutes. [02 MARKS III. Time it takes for the reaction to reach 80% of the maximum velocity, if the initial concentration of the substrate is ($1. 75 umol.L-? [06 MARKS]