Question

Consider the most common Cystic Fibrosis variant: Wild-type CFTR DNA (Coding-strand): 3 ATC ATC TTT GGT GTT atc att ggt gtt... Cystic Fibrosis odi 1. Add the 5' and 3' designations to the DNA. 2. Write the template sequence for the DNA 3. Transcribe the wild-type and CF DNA into mRNA. Be sure to include the 5'/3' labels. 4. Translate the wild-type and CF mRNA into protein. Be sure to label these ends also. 5. In the above wild-type protein, underline all of the amino acids that have nonpolar characteristics. Circle those that have polar characteristics. Draw the mutant peptide, including the specific R groups that are found with this sequence Circle one peptide bond.

Answer 1

1.

Wild type CFTR DNA- 3'-ATC ATC TTT GGT GGT-5'

Cystic fibrosis patient CFTR DNA- 3'ATC ATT GGT GTT-5'

2.

Template sequence for DNA:3'-ATC ATC TTT GGT GGT-5'

3.

5'AUCAUCUUUGGUGGU3'

4.

5'IIFGG 3'

5.

I I F G G