Question

The thermal denaturation of mystery protein X has a standard molar entropy of 512 ㎾mol and a standard molar entropy of 1.60 X 103 J/(K mol). Is the denaturation process spontaneous at 25 °C?

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Solution

The standard molar enthalpy of mystery protein = 512 kJ/mol

The standard molar entropy of mystery protein = 1.6 x 103 J/(K. mol)

Thus, the positive value of enthalpy of protein (+512 kJ/mol) indicates that the denaturation process is endothermic and requires an input of energy. This energy is required to be broken in order to denature the protein. The positive value of entropy of protein (+1.6 x 103 J/(K. mol)) indicates that the denaturation process of protein would increase of entropy. So, a denatured protein is less ordered than a natural protein.

So, the denaturation process of protein is not spontaneous at 25°C.

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