You are dissatisfied with the purity of the final preparation of your HRP and decided to include ion-exchange (IEC) and gel-exclusion chromatography (GEC) steps in your purification protocol. From the literature, you found out the following info about HRP enzyme:
Molecular weight: 40,000
Isoelectric point: 7.2
HRP is a very stable protein.
2: Design GEC purification experiment using the information in the appendix. Address and justify the following
I. choice of GEC column
II. choice of the buffer (if you do GEC following the IEC, can you use the same buffer?)
III. where do you expect your protein to elute depending on your chosen column separation range?
IV. suppose HRP tends to form dimers, would your column of choice be able to separate dimers and monomers?
Apendix
You are dissatisfied with the purity of the final preparation of your HRP and decided to...
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