What prevents oocyte to enter M phase?
I am working on a meiotic cell division using Xenopus oocyte. I have taken some genomic DNA and after shearing it through a needle, coated a 100 nm beads to act as chromosome to see what would happen if I have extra chromosome in oocyte.
when coated beads are injected into germinal vesicle of oocyte, and activate oocyte with progesterone, oocyte does not enter M phase. While, if I had uncoated beads, oocyte could go to M phase.
1-I want to know what is going on by drawing the pathway.?
2-what I may do to allow entering to M phase in case of coated beads?
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What is effect of loss of negative regulators of signal transduction on uncontrolled cell proliferation?
How loss of negative regulators of signal transduction result in uncontrolled cell proliferation in a cell that require a mitogen such as EGF to enter cell cycle?
1. If DNA is physically damaged, protein p53 activates genes that either stop cell cycle until damage is repaired and thats why oocyte doesnt enter M phase. Genomic DNA - sheared - coated onto beads- germinal vesicle - added progesterone.
2. If DNA is not damaged then it allows entering to M phase.
What prevents oocyte to enter M phase? I am working on a meiotic cell division using...