Membrane%20Receptors(1).pdf Study Questions One of the simple sugars (galactose or mannose) used in this exercise should...
Membrane%20Receptors(1).pdf Study Questions One of the simple sugars (galactose or mannose) used in this exercise should have inhibited Con A-peroxidase binding to cheek epithelial cells and the Con A-induced hemoagglutination reaction. Name this sugar and describe the mechanism by which it inhibits these effects. Describe the mechanism responsible for the Con-A induced hemoaggluti- nating reaction. Most membrane proteins including the Con-A receptor can be solubilized by treating cell membranes with a non-ionic detergent such as Triton X 100. You are given such a soluble preparation of erythrocyte proteins. Describe a single procedure that could be used to isolate the Con A Receptor from this preparation. Selected References Barondes, S.H. Lectins: Their Multiple Endogenous Cellular Factors, Ann, Rev. Biochem, 50:207-231, 1981. Zick, Y. The Insulin Receptor: Structure and Function, Critical Reviews in Biochemistry and Molecular Biology, Vol 24, pp 217-269, 1989.