Below are the Kcat and Km values for 5 hypothetical enzymes. Which enzyme would have the...
Below are the Kcat and Km values for 5 hypothetical enzymes. Which enzyme would have the largest specificity constant?
Enzyme A - Kcat = 2.6 x 103 , Km = 4 x 10-4
Enzyme B - Kcat = 9 x 102 , Km = 6.1 x 10-6
Enzyme C - Kcat = 1.7 x 10-3 , Km = 3.2 x 105
Enzyme D - Kcat = 2.6 x 10-7 , Km = 4 x 104
Enzyme E - Kcat = 800, Km = 0.03
Homework Answers
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Below are the Kcat and Km values for 5 hypothetical enzymes.
Which enzyme would have the...
please help!! please please "Looking at table 6-8 on Enzyme Reactions from the lecture, which achieves...
please help!! please please
"Looking at table 6-8 on Enzyme Reactions from the lecture,
which achieves an excellent specificity constant with a very
favorable kcat (insert answer here) , which with a
favorable Km (insert answer here) , and which with
moderate values for each (insert answer here) ?
(pick from acetyl cholinesterase, B-lactamase, and catalase)"
Question 2: "The units of Vmax are (insert answer
here) and the units of Km are (insert answer
here) (pick from M, M-1, M/s, s/M...
Values for an Enzyme and a substrate are: Km=4 uM and kcat=20/min. For an experiment where...
Values for an Enzyme and a substrate are: Km=4 uM and kcat=20/min. For an experiment where [S]=6mM, it was found that Vo=480nM/min. What was the enzyme concentration? Give your answer in nM. Using the same kcat and Km as the previous question, if [Et]=0.5uM gives a Vo=5 uM/min, what wat the [S]? Give your answer in uM. reaction is run with the kcat=20/min and the Km=4uM. Use the enzyme concentration from question 1 above. A very strong inhibitor is added creating...
only 7-9,11-12 7. When does the KM equal the Kd? 8. The kcat refers to which...
only 7-9,11-12
7. When does the KM equal the Kd? 8. The kcat refers to which steps in the catalytic mechanism? 9. The KM refers to which steps in the catalytic mechanism? L0. Calculate the Km, when v = .123 HM/sec, [E]T = 1.0 HM, kcat 720/s and [S 11. Which constant would you use to compare substrate specificities? 12. The upper limit of enzyme catalysis is called...?
7. When does the KM equal the Kd? 8. The kcat refers...
only 7-9,11-12 7. When does the KM equal the Kd? 8. The kcat refers to which...
only 7-9,11-12
7. When does the KM equal the Kd? 8. The kcat refers to which steps in the catalytic mechanism? 9. The KM refers to which steps in the catalytic mechanism? L0. Calculate the Km, when v = .123 HM/sec, [E]T = 1.0 HM, kcat 720/s and [S 11. Which constant would you use to compare substrate specificities? 12. The upper limit of enzyme catalysis is called...?
9. What is the volume of a kilogram of water? - 10. What is the weight...
9. What is the volume of a kilogram of water? - 10. What is the weight of five liters of water?_tx1000R o 11. Complete the following calculations. Give your answers in scientific notation: 1.0 X 106* 1.0 X 103 = 7.2 x 10-4 * 6.1 x 10-7 = 4.1 X 103 * 2.9 X 10-5 = 8.4 X 106/2.1 x 104 = 1.0 X 105/3.3 x 10-8 = 6.1 x 10-3/3.2 x 10-4 = [10** 10- ) *[104 * 10-s)....
4. For which of the following 0.010 M diprotic acids would the second dissociation affect the...
4. For which of the following 0.010 M diprotic acids would the second dissociation affect the pH significantly? Explain your answer. H2A; Kai = 4.2 x 102, K2 = 1.8 x 10-7 b. H2B; Kai = 2.4 x 104, K2 = 6.1 x 108 H2C; Kai = 1.3 x 104, K2 = 5.2 x 10-9 H2D; Kai = 1.8 x 10", K 2 = 9.3 x 104 c.
Refer to the below Enzyme activity data (Table 4) to determine the following: (1.5 pts each) a) V...
Refer to the below Enzyme activity data (Table 4) to determine the following: (1.5 pts each) a) Vmax b) why is the velocity v constant at [S] greater than 2 x 10-3 M? c) what is the free [E] at [S] = 2 x 10-2 M? 2. SI (mol/L 2 x 10-l 2 x 10-2 2 x 10-3 2 x 104 5 x 10-4 1,3 x 10-5 ol/min 60.00 60.00 60.00 48.00 45.00 2.00 Table 4. Enzyme activity assay data...
a. what are the values of Vmax and Km in the abscence if the inhibitor what...
a. what are the values of Vmax and Km in the abscence if the
inhibitor what are the values of Vmax and Km in the presence of the
inhibitor?
b. what type of inhibition is it?
c. what is the dissociation constant (Ki) of the
inhibition?
***d. graph a linear scatter plot including equation.
Homework (CHE 407) The initial velocity for an enzyme-catalyzed reaction is measured at various initial substrate concentration [S]o, in the absence and in the presence of...
8. Enzymes often exhibit pH-dependent activity due to protonation/deprotonation of key amino acids. A general schematic...
8. Enzymes often exhibit pH-dependent activity due to protonation/deprotonation of key amino acids. A general schematic is shown in i) for a pH-dependent enzyme, in which the "active" pathway requires EH and ESH. The constants ka, kz, kcat are the usual rate constants for the Michaelis-Menten reaction scheme, and the dissociation constants KEI, KESI, KE2, Kesz are defined as shown in ii). a. Derive a M-M rate equation for the pH-dependent scheme, below. To do so: 1) express total enzyme...
these were provided with the test Question 32 3 pts What is the molar solubility.s, of...
these were provided with the test
Question 32 3 pts What is the molar solubility.s, of a saturated solution of cadmium sulfide, CdS*? O 9.3 x 10-10 O 2.2 x 10-9 O2.7 x 10-18 O 7.9 x 10-8 O 8.4 x 10-13 lonization Constants for Acids at 25°C Name Formula Kai К2 Kaz Acetic Arsenic 1.0 x 107 3.0 x 10-12 Arsenous Ascorbic 1.6 x 10-12 Benzoic Boric HC2H302 H₂A504 НАО H2C8H08 HG,HgO2 H803 HC H102 H.CO, HC H20201 HCIOZ...
please help!! please please
"Looking at table 6-8 on Enzyme Reactions from the lecture,
which achieves an excellent specificity constant with a very
favorable kcat (insert answer here) , which with a
favorable Km (insert answer here) , and which with
moderate values for each (insert answer here) ?
(pick from acetyl cholinesterase, B-lactamase, and catalase)"
Question 2: "The units of Vmax are (insert answer
here) and the units of Km are (insert answer
here) (pick from M, M-1, M/s, s/M...
only 7-9,11-12
7. When does the KM equal the Kd? 8. The kcat refers to which steps in the catalytic mechanism? 9. The KM refers to which steps in the catalytic mechanism? L0. Calculate the Km, when v = .123 HM/sec, [E]T = 1.0 HM, kcat 720/s and [S 11. Which constant would you use to compare substrate specificities? 12. The upper limit of enzyme catalysis is called...?
7. When does the KM equal the Kd? 8. The kcat refers...
only 7-9,11-12
7. When does the KM equal the Kd? 8. The kcat refers to which steps in the catalytic mechanism? 9. The KM refers to which steps in the catalytic mechanism? L0. Calculate the Km, when v = .123 HM/sec, [E]T = 1.0 HM, kcat 720/s and [S 11. Which constant would you use to compare substrate specificities? 12. The upper limit of enzyme catalysis is called...?
9. What is the volume of a kilogram of water? - 10. What is the weight of five liters of water?_tx1000R o 11. Complete the following calculations. Give your answers in scientific notation: 1.0 X 106* 1.0 X 103 = 7.2 x 10-4 * 6.1 x 10-7 = 4.1 X 103 * 2.9 X 10-5 = 8.4 X 106/2.1 x 104 = 1.0 X 105/3.3 x 10-8 = 6.1 x 10-3/3.2 x 10-4 = [10** 10- ) *[104 * 10-s)....
4. For which of the following 0.010 M diprotic acids would the second dissociation affect the pH significantly? Explain your answer. H2A; Kai = 4.2 x 102, K2 = 1.8 x 10-7 b. H2B; Kai = 2.4 x 104, K2 = 6.1 x 108 H2C; Kai = 1.3 x 104, K2 = 5.2 x 10-9 H2D; Kai = 1.8 x 10", K 2 = 9.3 x 104 c.
Refer to the below Enzyme activity data (Table 4) to determine the following: (1.5 pts each) a) Vmax b) why is the velocity v constant at [S] greater than 2 x 10-3 M? c) what is the free [E] at [S] = 2 x 10-2 M? 2. SI (mol/L 2 x 10-l 2 x 10-2 2 x 10-3 2 x 104 5 x 10-4 1,3 x 10-5 ol/min 60.00 60.00 60.00 48.00 45.00 2.00 Table 4. Enzyme activity assay data...
a. what are the values of Vmax and Km in the abscence if the
inhibitor what are the values of Vmax and Km in the presence of the
inhibitor?
b. what type of inhibition is it?
c. what is the dissociation constant (Ki) of the
inhibition?
***d. graph a linear scatter plot including equation.
Homework (CHE 407) The initial velocity for an enzyme-catalyzed reaction is measured at various initial substrate concentration [S]o, in the absence and in the presence of...
8. Enzymes often exhibit pH-dependent activity due to protonation/deprotonation of key amino acids. A general schematic is shown in i) for a pH-dependent enzyme, in which the "active" pathway requires EH and ESH. The constants ka, kz, kcat are the usual rate constants for the Michaelis-Menten reaction scheme, and the dissociation constants KEI, KESI, KE2, Kesz are defined as shown in ii). a. Derive a M-M rate equation for the pH-dependent scheme, below. To do so: 1) express total enzyme...
these were provided with the test
Question 32 3 pts What is the molar solubility.s, of a saturated solution of cadmium sulfide, CdS*? O 9.3 x 10-10 O 2.2 x 10-9 O2.7 x 10-18 O 7.9 x 10-8 O 8.4 x 10-13 lonization Constants for Acids at 25°C Name Formula Kai К2 Kaz Acetic Arsenic 1.0 x 107 3.0 x 10-12 Arsenous Ascorbic 1.6 x 10-12 Benzoic Boric HC2H302 H₂A504 НАО H2C8H08 HG,HgO2 H803 HC H102 H.CO, HC H20201 HCIOZ...
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