Read the following abstract:
"The advent of high-throughput sequencing led to breakthroughs in microbiome analysis and to an explosion of bacterial genomes being released in public repositories. However, many microbiotas cannot be cultured in the lab without introducing drastic changes in the underlying bacterial communities and the distribution of genomes present in online repositories is heavily skewed towards organisms that are amenable to culturing. Those that cannot (or should not in the case of dangerous pathogens) be cultured are often underrepresented and culture-free methods require sequencing technologies capable of starting from tiny amounts of input DNA. While Illumina libraries starting from 1 ng of DNA have been available for a few years, investigating microbiomes using short reads is difficult. Bacterial genomes sequenced with this platform are also often incomplete due to the presence of repetitive elements. Longer sequencing reads such as those provided by the PacBio platform are very valuable when investigating complex bacterial communities and genomes, but the amounts of material required by this platform (in the 5-10 ug range) limits what can be done with it. One of the appeals of the latest long read technology from Oxford Nanopore is that it can starts from minute amounts of DNA. We used 16S amplicon-based nanopore sequencing to investigate diseased microbiomes and developed a quick pipeline to analyze the resulting data. We also used a hybrid illumina/nanopore approach to sequence various staphylococcal and streptococcal species from limited amounts of genomic DNA. Both approaches are simple enough to be performed by undergraduates with limited supervision."
Write an in depth question (do not answer it) to the author of this abstract concerning the subject matter.
What could be the safer method for sequencing the microbiomes without using large amount of genomes. What is the need for a safe method? Give an account on all the methods previously done and the latest techniques used by using limited amounts of genomic DNA?
Read the following abstract: "The advent of high-throughput sequencing led to breakthroughs in microbiome analysis and...
Please read the article bellow and discuss the shift in the company's approach to genetic analysis. Please also discuss what you think about personal genomic companies' approaches to research. Feel free to compare 23andMe's polices on research with another company's. Did you think the FDA was right in prohibiting 23andMe from providing health information? These are some sample talking points to get you thinking about the ethics of genetic research in the context of Big Data. You don't have to...