Question

4) Draw the titration curves for the amino acids Lysine and Alanine. Be sure to label your axes. (5 pts) a) Identify the pKi, pK and pKR (if relevant) for each amino acid. b) Identify the buffering region around pKR for arginine c) Identify the pl of each amino acid d) Draw the structure of the primary chemical species in each pH region of the titration curve.
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Answer #1

Titration curve for lysine

Lys2+ Lys Lys Lys COO COO COOH COO CH2 CH2 CH2 CH2 NH3+ CH2 CH2 CH2 CH2 NH2 CH CH2 CH2 CH2 CH2 NH3+ 2 CH2 CH2 CH2 NH3 14 12 pK3 10 pk2 point pH pKy 2.0 3.0 1.0 0 Equivalents of OH added

For Alanine

Alanine 14.0 12.0 10.0 8.0 6.0 4.0 2.0 12:0 10.0 8.0 6.0 CO2 pk 2-9.69 H2N CH3 pH> 10 pH CO2 p12601 H3N CH3 pKa1-2.34 CO2H pH

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