Question

Describe what has gone with TLC-plates below:

Answer 1

Solution :-

For the TLC plate A the one spot is placed on lower side of the base line which may get dissolved in the solvent mixture and may not run well on the TLC plate.

For the TLC plate B the both compound spots are run along with the solvent and reaches to the solvent front line therefore the Rf value cannot be calculated for them.

For the TLC plate C the spots were well separated and run properly so that the Rf value of the components can be calculated. But the TLC plate might be placed tilted in the developing chamber that is why the spots are run and appear little bent towards right side.

For the TLC plate D the spots are very large that is why they got mixed with one another so we cannot distinguish them and hence the Rf value cannot be calculated for them