Homework Answers
You have TLC which shows distance traveled by solvent Indicated by pencil mark above from the pencil mark of the bottom line where you spoted your spots.
You can calculate the distance traveled by each spot from the bottom line where you put spot.
Calculate distance traveled by Solvent in mm
Calculate distance traveled by each spot separately in mm and then. You can calculate Rf value with the formula
Rf = Distance traveled by spot 1 /distance traveled by solvent
With this you can calculate Rf of each spot. See attached TLC
picture
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CAN SOMONE PLEASE HELP. above is a picture of two TLC plates
with spots on them....
Possible Quiz Questions 1 What two methods of visualizing spots on a TLC plate will you...
Possible Quiz Questions 1 What two methods of visualizing spots on a TLC plate will you use in this Give two reasons why would you predict that the elution solvents (hexanes or acetate) would not be visible under UV visualization? week's lab? ethyl 2. Define R 3. Calculate the R of the spot on the following TLC plate. 4. Which compound in each pair is predicted to have the higher Ri? a. CH3CH2CH2CH2OH and CH3CH2CH2CH28r b. CH3CH2CH2CH2OH and CH3CH2CH2CH2CH2CH2CH3 c....
Please help 2. Consider the TLC plate shown below that was done on silica gel using...
Please help
2. Consider the TLC plate shown below that was done on silica gel using a solvent mixture of 4:1 hexane/EtoAc as the eluent. 4:1 Hexane:EtOAC marked solvent front Spot B Spot A A origin (a) Which of the two organic compounds labeled A and B is less polar? (b) Calculate the Rf value of compound B. (c) How would the following changes affect the Rf values of A and B? i. Change to a more polar solvent mixture...
Can someone please help me out with the Rf value PLEASE!! Thin Layer Chromatography Procedures 1)...
Can someone please help me out with the Rf value
PLEASE!!
Thin Layer Chromatography Procedures 1) Prepare a solvent solution in a 150 mL beaker by using 5 mL of Hexane and 3 mL of ethyl acetate Take one plate and spot (small spots) it with the three different standard solutions given. The standard solutions are 2-nitroaniline, 3-nitroaniline, and 4-nitroaniline. The fourth spot will be the unknown sample obtained. 2) 3) Align these spots at 1 cm from the bottom...
Two compounds might have spots with the same Rf value in a TLC experiment and not...
Two compounds might have spots with the same Rf value in a TLC experiment and not be identical compounds. Which proposed TLC experiment could best help determine whether the compounds are identical? Select one: Repeat the original experiment with a mixture of the compounds in a single spot Repeat the original experiment with larger spots of each compound Repeat the original experiment with a different solvent system Repeat the original experiment with the development chamber jar open
I need help with my pre and post lab questions. The three compounds below were spotted...
I need help with my pre and post lab questions.
The three compounds below were spotted on a TLC plate and developed to give the TLC plate shown. Which compound corresponds to which spot? Calculate the Rf value for spot B in the TLC plate shown above. When using polarity to determine the relative rates at which organic compounds travel up the TLC plate, we are considering compounds of similar molecular weight. It should also be noted that Rf values...
3. If the TLC plate development had stopped before the solvent front reached the top of...
3. If the TLC plate development had stopped before the solvent front reached the top of the TLC plate (i.e. maybe it reached the middle of the TLC plate when it was stopped, instead): a) Would the Rf values have been affected? If so, how? If not, why not? Give a numerical example to support your answer. b) Would the size of the spots have been affected? If so, how? If not, why not? (Hint: think about the diffusion of...
please do two pargraph about this pre lab and lab include Title,purpose ,chemical eqution if their...
please do two pargraph about this pre lab and lab include
Title,purpose ,chemical eqution if their any ،Procedure
,Hazards(hazard Identification,physical and chemical
properties),Experimental Phase, Data
&Observations,Data&Observations cont (melting point and
meling point). and Calcuations
Spinach Laboratory Procedure PROCEDURES Isolation of pigment from leaves: 1. Wash the spinach leaves. Weigh 1.0 g-2.0 g of dry spinach leaves. Remove any stems and veins from the leaves before you weigh. 2. Combine 1.0 g of anhydrous magnesium sulfate with 0.5 g of sand...
Results: Distance of the solvent front in mm: 55 mm Compound acetanilide acetylsalicylic acid acetaminophen ibuprofen...
Results: Distance of the solvent front in mm: 55 mm Compound acetanilide acetylsalicylic acid acetaminophen ibuprofen phenacetin Solute distance R value colour of spot / observations Saturated purple colour 0.55 Elongated purple colour 22 mm 30 mm 6 mm 47 mm 12 mm 0.40 Saturated purple colour 0.85 Elongated purple colour Saturated purple colour 0.11 0.22 Unknown #-5 Compound compound 1 compound 2 contains the following compounds: Solute distance Rf value colour of spot / observations 0.22 Saturated purple colour...
Case C Thin Layer Chromatography 1. TOXI-LAB is a screening method that uses: chromatography (column /...
Case C Thin Layer Chromatography 1. TOXI-LAB is a screening method that uses: chromatography (column / planar) The first step consists of the extraction of drug metabolites from biological fluids when we place the sample in a test tube containing a mixture of solvents and buffering salts that cause the extraction of basic and neutral drugs. The solvent extract of the sample is concentrated by heat and evaporation, then is deposited onto a small disc of chromatographic media (small circle...
can u help me with 1,2,3? 1. (2 pts - 1 pt for correct traveling order,...
can u help me with 1,2,3?
1. (2 pts - 1 pt for correct traveling order, 1 pt for explanation) Based on the skeletal structures of biphenyl, benzhydrol and benzophenone, for a given TLC eluent, which of the three compounds would you expect to travel further on the TLC plate, and why? Copy your prediction into the Introduction section of your lab notebook. 2. (1 pt -0.5 pts per spot) Calculate the retention factor (R1) for each of the spots...
Possible Quiz Questions 1 What two methods of visualizing spots on a TLC plate will you use in this Give two reasons why would you predict that the elution solvents (hexanes or acetate) would not be visible under UV visualization? week's lab? ethyl 2. Define R 3. Calculate the R of the spot on the following TLC plate. 4. Which compound in each pair is predicted to have the higher Ri? a. CH3CH2CH2CH2OH and CH3CH2CH2CH28r b. CH3CH2CH2CH2OH and CH3CH2CH2CH2CH2CH2CH3 c....
Please help
2. Consider the TLC plate shown below that was done on silica gel using a solvent mixture of 4:1 hexane/EtoAc as the eluent. 4:1 Hexane:EtOAC marked solvent front Spot B Spot A A origin (a) Which of the two organic compounds labeled A and B is less polar? (b) Calculate the Rf value of compound B. (c) How would the following changes affect the Rf values of A and B? i. Change to a more polar solvent mixture...
Can someone please help me out with the Rf value
PLEASE!!
Thin Layer Chromatography Procedures 1) Prepare a solvent solution in a 150 mL beaker by using 5 mL of Hexane and 3 mL of ethyl acetate Take one plate and spot (small spots) it with the three different standard solutions given. The standard solutions are 2-nitroaniline, 3-nitroaniline, and 4-nitroaniline. The fourth spot will be the unknown sample obtained. 2) 3) Align these spots at 1 cm from the bottom...
I need help with my pre and post lab questions.
The three compounds below were spotted on a TLC plate and developed to give the TLC plate shown. Which compound corresponds to which spot? Calculate the Rf value for spot B in the TLC plate shown above. When using polarity to determine the relative rates at which organic compounds travel up the TLC plate, we are considering compounds of similar molecular weight. It should also be noted that Rf values...
please do two pargraph about this pre lab and lab include
Title,purpose ,chemical eqution if their any ،Procedure
,Hazards(hazard Identification,physical and chemical
properties),Experimental Phase, Data
&Observations,Data&Observations cont (melting point and
meling point). and Calcuations
Spinach Laboratory Procedure PROCEDURES Isolation of pigment from leaves: 1. Wash the spinach leaves. Weigh 1.0 g-2.0 g of dry spinach leaves. Remove any stems and veins from the leaves before you weigh. 2. Combine 1.0 g of anhydrous magnesium sulfate with 0.5 g of sand...
Results: Distance of the solvent front in mm: 55 mm Compound acetanilide acetylsalicylic acid acetaminophen ibuprofen phenacetin Solute distance R value colour of spot / observations Saturated purple colour 0.55 Elongated purple colour 22 mm 30 mm 6 mm 47 mm 12 mm 0.40 Saturated purple colour 0.85 Elongated purple colour Saturated purple colour 0.11 0.22 Unknown #-5 Compound compound 1 compound 2 contains the following compounds: Solute distance Rf value colour of spot / observations 0.22 Saturated purple colour...
Case C Thin Layer Chromatography 1. TOXI-LAB is a screening method that uses: chromatography (column / planar) The first step consists of the extraction of drug metabolites from biological fluids when we place the sample in a test tube containing a mixture of solvents and buffering salts that cause the extraction of basic and neutral drugs. The solvent extract of the sample is concentrated by heat and evaporation, then is deposited onto a small disc of chromatographic media (small circle...
can u help me with 1,2,3?
1. (2 pts - 1 pt for correct traveling order, 1 pt for explanation) Based on the skeletal structures of biphenyl, benzhydrol and benzophenone, for a given TLC eluent, which of the three compounds would you expect to travel further on the TLC plate, and why? Copy your prediction into the Introduction section of your lab notebook. 2. (1 pt -0.5 pts per spot) Calculate the retention factor (R1) for each of the spots...
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