QUESTION: In the eve-stripe 2 enhancer, predict the effect of mutating the Kr (orange) binding sites given the preceding information (both expression graphs).
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QUESTION: In the eve-stripe 2 enhancer, predict the effect of
mutating the Kr (orange) binding sites...
Consider cross-regulatory interactions of Drosophila gap genes (“+” and “-“ reflect positive and negative interactions. Predict...
Consider cross-regulatory interactions of Drosophila gap genes
(“+” and “-“ reflect positive and negative interactions. Predict
the effect of the following conditions on gene expression domains –
specify effect on both anterior and posterior expression
boundaries.
hint: for each gap gene domain, consider anterior and
posterior boundary separately
----In the eve-stripe 2 enhancer, predict the effect of
mutating the Kr (orange) binding sites
hb 100% 90% a0 % 70% 60% 50% 40% 30% 2 % 10%EL hb gt stripe Kr...
The Drosophila regulatory segment that defines the location of Eve stripe 2 contains binding sites for...
The Drosophila regulatory segment that defines the location of Eve stripe 2 contains binding sites for four different transcription regulators: two repressors (Giant and Krüppel) and two activators (Bicoid and Hunchback). For Eve to be efficiently expressed in stripe 2, both repressors must be absent and both activators present. What would you expect to see in flies that lack the gene that encodes Bicoid? (Assume that Bicoid does not influence the expression of Hunchback, Giant, or Krüppel.) Giant Krüppel stripe...
1 & 2) Consider cross-regulatory interactions of Drosophila gap genes (“+” and “-“ reflect positive and...
1 & 2) Consider cross-regulatory interactions of Drosophila
gap genes (“+” and “-“ reflect positive and negative interactions.
Predict the effect of the following conditions on gene expression
domains – specify effect on both anterior and posterior
expression boundaries.
hint: for each gap gene domain, consider anterior and
posterior boundary separately
a) loss of function of (zygotic) hb on kni expression
ho 100 % 90% a0% 70% 60% 50% 40% 30 % 20% 10% EL
Consider cross-regulatory interactions of Drosophila gap genes
(“+” and “-“ reflect positive and negative interactions. Predict
the effect of the following conditions on gene expression domains –
specify effect on both anterior and posterior expression
boundaries.
hint: for each gap gene domain, consider anterior and
posterior boundary separately
----In the eve-stripe 2 enhancer, predict the effect of
mutating the Kr (orange) binding sites
hb 100% 90% a0 % 70% 60% 50% 40% 30% 2 % 10%EL hb gt stripe Kr...
The Drosophila regulatory segment that defines the location of Eve stripe 2 contains binding sites for four different transcription regulators: two repressors (Giant and Krüppel) and two activators (Bicoid and Hunchback). For Eve to be efficiently expressed in stripe 2, both repressors must be absent and both activators present. What would you expect to see in flies that lack the gene that encodes Bicoid? (Assume that Bicoid does not influence the expression of Hunchback, Giant, or Krüppel.) Giant Krüppel stripe...
1 & 2) Consider cross-regulatory interactions of Drosophila
gap genes (“+” and “-“ reflect positive and negative interactions.
Predict the effect of the following conditions on gene expression
domains – specify effect on both anterior and posterior
expression boundaries.
hint: for each gap gene domain, consider anterior and
posterior boundary separately
a) loss of function of (zygotic) hb on kni expression
ho 100 % 90% a0% 70% 60% 50% 40% 30 % 20% 10% EL
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