Question

Lysozyme is an anti-microbial enzyme that cleaves the bonds of sugars that exist in the bacterial cell wall. 7. (15plu.) A model for the lysozyme-catalyzed reaction is below and on the supplemental information sheet. HO Ets ES *EP2 P2 = *EP2 + P** - Ep2** E + P2 HO For steps i-v in the lysozyme mechanism (forward direction, only), match the description of what is happening to the step of the chemical equation schematic by writing the letter of the description next to the enzymatic step. Descriptions The enzyme forms a covalent bond with the second product and is non-covalently bound to the first product. The second product is released by the enzyme. The free enzyme and free substrate come together to form the non-covalent enzyme substrate complex The enzyme releases the first product, but is still covalently bound to the second product. Water hydrolyzes the covalent bond between the enzyme and the second product. The second product remains non-covalently bound to the enzyme. Enzymatic Step Matching Description Enzymatic Step Matching Description H20 E+S = ES iv. E.P2 E-P2 + P1 HO E S = E-P2.P1 E.P2=E+ P2 E-P2.P1 = E-P2 + P1

Answer 1

Matching of the descriptions of what is happening to the step of the chemical equation schematic with the letter of the description box are as follows:

i	C
ii	A
iii	D
iv	E
v	B