Question

Assignment 1. - Word File Insert Design Layout References Mailings Review View Tell me what you want to do Sign in Share Home Cut Find- Aria E Copy Paste Select Editing Format Painter Clipboard Font Paragraph Styles PANCREAS LENt NEURAL TUBE RETINA CORNEA 3 4 イ乙 5. (2) The diagram above shows the a gene with its regulatory sequences. Exon sequences are orange, and enhancer sequences are green, the promoter is red. The enhancers direct gene expression differentially in the pancreas, the lens and cornea of the eye, the retina, and the neural tube. One of the ways that is used to determine what enhancer sequences are required for tissue specific expression is with reporter genes. The embryo on the right is an example of this type of experiment where beta-galactosidase activity is detected in the lens/cornea and in the pancreas. Draw a diagram showing the DNA construct that musthave been incorporated into all the cells of the embryo to obtain that pattern of staining Diagram $ galactoidue Page 2 of 7 1124 words English Canada) 甲 + 130% S:45 PM O Type here to search rP ^ ヨ瘟脈4x ENG 2018-10-05

Answer 1

That's simple.

To see which enhancer is active at what site, we just need to put the beta galactosidase gene with the individual promoters, in different plasmids. For example, here we need four types of constructs, each having one enhancer sequence (and obviously, the promoter sequence) upstream to the beta-galactosidase coding gene. If the enhancer is active, it will cause beta-galactosidase to express, which will produce blue colour in presence of X-gal.

When we do not know the functions of enhancers, we need to express such kinds of constructs in different embryos and see which sites produce blue colour, to know which sites the enhancer is active in. Since only the lens/cornea and pancreas get blue colour, the construct should be like this -

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