Give a brief procedure on how to perform a standard addition using the graphical method. Assume...
Give a brief procedure on how to perform a standard addition using the graphical method. Assume you are expected to run 5 samples and that the analyte has a uv absorption. For the plot, what do the x and y axes represent and how do you find the concentration of the unknown from the plot?
Homework Answers
First, there are 5 aliquots of the unknown solution in 5 containers, different volumes of standard solution are added with the same analyte and known concentration to the recipients, leaving one of them without a standard solution and all diluted to the same volume with water.
The UV Absorption value is taken from each sample and the UV Absorption value on the Y axis and the concentration value on the X axis are plotted.
The best line is obtained with an equation of the form:
Y = m * X + b
To obtain the concentration of the unknown, it is assumed that Y = 0 and the equation of the line is cleared, having:
X = b / m
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