Question

A solution of 0.181 M cysteine is titrated with 0.0453 M HNO3. The pKa values for cysteine are 1.70, 8.36, and 10.74, corresponding to the carboxylic acid group, thiol group, and amino group, respectively. Calculate the pH at the equivalence point. NH3 Number pH 6.22 CH- CH2 SH Cysteine

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Answer #1

pI is the pH at isoelectric point.
Only those pka are taken at which post protonation/ de-protonation no charge remains. For here the pKa are 1.70, 10.74
pI=1/2(1.70+10.74)
pI=6.22

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