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In this particle you took two samples of you original ground leaf or root sample to...

In this particle you took two samples of you original ground leaf or root sample to use as replicates for the estimate of soluble carbohydrate stored as starch.Is this a good way to obtain replication?Is there a better way to obtain replicate measures for carbohydrate in leaves and roots.
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Answer #1

You took two samples of your original ground leaf or root sample means you have taken two different leaves or two different roots. Hence the amount of soluble carbohydrate or starch present in each will be different. So this is not a good method to take replicates.

First you have to take only one leaf or root. Then find out the soluble carbohydrate in that leaf or root. Prepare its homogenate. From that homogenate supernatant,, collect the same volume in 3 different test tubes and perform the test for carbohydrate. Then you get 3 different values which are replicates for the same test.

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