TBE Buffer (Tris Borate EDTA) is prepared in a 5X Stock for storage, then diluted to...

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TBE Buffer (Tris Borate EDTA) is prepared in a 5X Stock for storage, then diluted to...

TBE Buffer (Tris Borate EDTA) is prepared in a 5X Stock for storage, then diluted to 1X for use. The 5X stock consits of 250mM Tris base, 250mM Boric Acid and 2.5mM EDTA.

Calculate the mass of EDTA (mw=372.24) required for 500mL of the 5X stock.

please explain in detail, thanks

science chemistry

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Answer 1

Answer #1

In 5X stock 250 mM is present

2.5 milli moles per Liter solution.

for 500 ml solution ( 2.5) * (500/1000) milli moles

mass of EDTA = 1.25 * 10-3 * 372.24

= 0.4653 g of EDTA

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Answer 2

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If you needed to make 500 mL of a TBE buffer (containing 0.1M Tris base, 0.1M Boric...

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TBE Buffer (Tris Borate EDTA) is prepared in a 5X Stock for storage, then diluted to...