TBE Buffer (Tris Borate EDTA) is prepared in a 5X Stock for storage, then diluted to 1X for use. The 5X stock consits of 250mM Tris base, 250mM Boric Acid and 2.5mM EDTA.
Calculate the mass of EDTA (mw=372.24) required for 500mL of the 5X stock.
please explain in detail, thanks
In 5X stock 250 mM is present
2.5 milli moles per Liter solution.
for 500 ml solution ( 2.5) * (500/1000) milli moles
mass of EDTA = 1.25 * 10-3 * 372.24
= 0.4653 g of EDTA
TBE Buffer (Tris Borate EDTA) is prepared in a 5X Stock for storage, then diluted to...
If you needed to make 500 mL of a TBE buffer (containing 0.1M Tris base, 0.1M Boric Acid, and 0.02M EDTA) how much of each of the following would you use: solid Tris base (121.1 g/mol); solid boric acid (61.8 g/mol); liquid stock solution of 0.50M EDTA?
1) How would you prepare 500mL of working TBE buffer if the stock solution was purchased as 25X TBE? 2) 2mL of a serum sample was added to 18mL of phosphate buffered saline (PBS) in Tube 1. 10mL of Tube 1 was added to 40mL of PBS in Tube What is the dilution of serum in Tube 2? 3) A student needed to prepare 500mL of 1X TAE buffer to run a QC gel. The stock solution in the lab...
solve for gel preparation and PCR mastermix calculations with steps for understanding TBE Buffer Calculations Determine the mass of the following reagents for a 10X stock 700mM of Tris Base (157g/mol) 887mM of Boric Acid (62g/mol) 25.7mM of EDTA (292g/mol) Dissolve in 750ml of DIH.O and bring to volume (IL) Calculate the dilution of your 10x stock for a 1X working stock. Remember you only need IL of working stock for a single experiment. Gel Preparation Calculations You need to...
please hlep me answer those three questions asap. Please ignore question 7. Question 7 Working with buffers You need 100 ml of 120 mM phosphate buffer and you have two stock solutions from which you can make the buffer; 0.6 M NaH2PO4 (the acid form) and 0.6 M Na2HP04 (the base form). You need to add 3 times more of the base form than the acid form to achieve the desired pH. How will you make this solution? Question 8...