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Presently I am working on metagenomics of coal biomethenation by bacterial consortium. I have got the...

Presently I am working on metagenomics of coal biomethenation by bacterial consortium.

I have got the sequence result (Illumina). The sequence is huge and I can't predict anything from the sequence. I have gone through different database like Metacyc, Biocyc etc. Please help me that how can I draw an inference towards the metabolic enzyme that are involved in biomethenation of coal.

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Answer #1

The first step after sequencing is finding probable genes. After that, genes and their proteins can be classified to belong to protein classes. This is the most what you can do with completely unknown genes. It's possible nowadays to predict the final structure using contact maps (if there is no homologous structure known) but this will still leave you unclear on ligands in many cases. So, the final step is to clarify the function with biochemical lab methods.

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