Question

Two loci are considered. Locus A is a length polymorphism when amplified by a pair of primers yielding either a 500 bp allele (A1) or a 1100 bp allele (A2). Locus B is a polymorphism that can be cut by restriction digest using Hindlll enzyme. PCR amplification yields either allele B1 that does not have a cut site and is 807 bp or allele B2 which has a cut site and breaks into two segments of 101 and 706 bp after digestion. Fill out the genotype table shown below with correct genotype inferred from the gel image.

Answer 1

If the corresponding allele is present it will show the band on the gel.

If 1100 bp band is there means it has A2 allele

If 807 bp bands is there means it has B1 allele

If 500 bp band is there means it has A1 allele

If 1100 bp band is there means it has A2 allele

If 706 bp band is there means it has B2 allele

If 101 bp band is there means it has B2 allele

1	2	3	4	5
A1A2B2B2	A1A1B1B1	A2A2B1B2	A1A2B2B2	B2B2
6	7	8	9	10
A1A2B1B2	A1A1B1B1	A1A1B1B2	A1A1B1B1	A2A2B2B2