Question

Can someone reword this for me? Please and thank you!

Cellular respiration is a process that converts energy from food molecules into the energy of ATP. It involves three stages that occur in the cytoplasm of cells and in the mitochondria. One of the three stages is the citric acid cycle which is a series of reactions that generates reduced electron carriers that transport electrons to the electron transport chain. Since the primary function of the mitochondria is to produce ATP, the citric acid cycle most likely occurs in the mitochondria. To test this hypothesis, the cellular components from cauliflower, namely the nuclei and the mitochondria, were separated using differential centrifugation. The oxidation of succinate to fumarate catalyzed by succinate dehydrogenase is a reaction that is part of the citric acid cycle. This reaction was utilized along with an artificial electron acceptor DCIP. When reduced, DCIP turns from a blue/violet color to a clear solution. DCIP absorbs light most efficiently as 410 nm and the change in absorbance over time was used to show that the citric acid cycle occurs in the mitochondria.

Answer 1

The process by which break down of food occurs and convert them into energy in the form of ATP is known as cellular respiration. In this process there are three stages which are found in the cytoplasm and mitochondria.

Citric acid cycle is one of the three stages. It produced reduced molecule which transfer electron to electron transport chain. This reaction takes place in the matrix of mitochondria.

For finding of our hypothesis, we separated mitochondria and nucleus from cauliflower by means of centrifugation. We have taken the reaction in test which includes conversion of fumarole from succinct by the process of oxidation in the presence of succinct dehydrogenase enzyme and we used DCIP as an artificial electron acceptor. When it takes electron, solution becomes white from blue. This change is detection at 410 nm absorbance in spectrophotometer. In this way we can tell about the rate of citric acid cycle and also tells that it occurs in mitochondria.