Question

For biochemistry:

Number 5 please, as much detail as possible would be great for me to better understand!

5. In order to understand the mechanism for ethanol production, the investigators measured the activity of the alcohol dehydrogenase (ADH) enzyme in both the wild type and transgenic tomatoes at each stage of the fruit-ripening process. The results are shown in Figure 12.4. What factor or factors might influence ADH activity in the ripening tomato? 0.5 0.4 0.3 0.2 Developmental Stage Figure 12.4: Alcohol dehydrogenase activity in ripening wild-type and transgenic tomato fruit (based on Frenkel, et al., 1998).

Answer 1

Simply an ethlene responsive element in the transgenic tomato fruit specific promoter sequence might be the reason ... as fruit ripens more ethylene is produced and that binds to ethylene responsive site in promoter and increase transcript expression of the transgene (adh) .... compared to wildtype .... hope it helps