What is the formula to evaluate the polarity index of a binary solvent mixture in reverse phase HPLC?
What is the formula to evaluate the polarity index of a binary solvent mixture in reverse...
Polarity index is a value indicative of the polarity of a compound (higher polarity index means more polar). Compare the polarity index of acetone, ethyl acetate, and hexane (available via the solvent miscibility table on Blackboard). Use your uncanny knowledge of chemical structures to evaluate the relative polarity of caffeine, aspirin, and acetaminophen. Use this information to predict which compound is likely to elute with each of the solvents employed in your chromatographic separation.
Huie Turva polar better with higher solvent stationary phase Study Problem: A mixture of organic compounds was analyzed by TLC using different solvent mixtures as mobile pha and the results are presented in the diagram below. Compare the TLC plates A-D with the one on the left and answer th questions: a) How is the polarity of the mobile phase used to TLC plate A comparative with plate on the left? b) How are the Ri's value on the plate...
4) What is the order of elution of the following compounds from a reverse-phase packed HPLC column? Briefly justify your reasoning. Benzyl alcohol (C6H5CH2OH), benzaldehyde (C6H5CHO), benzoic acid (C6H5COOH), Acetophenone (C6H5C(O)CH3)
4) (10 pts) What is the order of elution of the following compounds from a reverse-phase packed HPLC column? Briefly justify your reasoning. Benzyl alcohol (C.HSCH-OH), benzaldehyde (C6HSCHO), benzoic acid (C6HCOOH), Acetophenone (C6H5C(O)CH3)
QUESTION 9 The index of the right child of a given node, X, in a binary heap is given by what formula? O index(X) * 2 + 1 index(X)* 2 index(X)* 2-1 index(X) + 1* 2 QUESTION 10 The worst case running time for adding an item to a binary heap is: O 0(1) O(NlogN) O(logN) O(N)
What is the main purpose of running a column chromatography? Similar to TLC. column chromatography is based on analytes being partitioned between a stationary phase (not moving) and a mobile phase (moving). What is most commonly used as stationary phase in a column? Is this material polar or non-polar? In this experiment, you will use different solvents to run column chromatography separation of different pigments extracted from spinach leaves. These solvents are a mixture of acetone and hexanes. (a) What...
1. From the phase diagram for a binary mixture of titanium and nickel, find: a. label the two-phase regions with the phases present in each case. b. The phase or phases present in a mixture of 2g nickel with 48g titanium @ 1000C c. Amount of additional nickel in g required for the alloy to start to form a liquid at 1000C. d. Composition of the first liquid that starts to form as nickel is added to this alloy e....
A solute A is being transferred from a binary gas mixture of A and C into a pure liquid solvent B in a counter current packed tower with a cross sectional area of 0.2 m2. The inlet gas contains 5 mol% of A and its desired to recover 87% percent of A from the gas stream. The feed gas flowrate is 3.7 mol/s and the liquid solvent inlet flowrate is 13 mol/s. ky’ah = 40 mol/m3s and kx’ah = 60...
What is the index of hydrogen deficiency of a compound with the molecular formula of CgH3Br5O2? on w N les
D Question 14 3.5 ts What is the line of solvent that moves up the TLC plate referred to as? O Solvent front O Capillary front Resolving factor Chromatographic boundary Question 15 3.5 pts Most organic compouds are colorless or white. A UV lamp assists in seeing your separation on TLC plates by: Making the organic sample spots glow and leaving the stationary phase dark Making the stationary phase glow, leaving dark spots where your sample is located Showing how...