Question

6a) During experiment 7, you performed column chromatography in order to separate two main compounds from tomato paste. What are these two compounds? 6b) Is this separation technique a chemical or physical method of separation? 6c) What color is each of these compounds? What about their molecular structure gives rise to their color? 6d) During the experiment, we only observed one band on the column even though we should have observed two. However, you may have noticed, we had in fact drained a lightly colored liquid from the column early on in the separation process. This means that we completely missed the first band due to it being so lightly colored. Since this lightly colored band came off of the column first, is the compound within this band more nonpolar or more polar than the compound within the other band still in the column?

Answer 1

6.

a. Lycopene and  $\beta$ - Carotene.

b. Physical Method.

c. Lycopene - Red & $\beta$ - Carotene - Orange ;

They Both are Isomers of C40H56.

d. 1st elated compound is $\beta$ - Carotene and the latter is Lycopene ;

  $\beta$ - Carotene is Less Polar Than Lycopene.

Lycopene B- carobene - Responsible Responsible for red colour for bange colour column chromatography is physical mothed of separation - As we are not Changing any compound by reaction. dycopene - Red colour 2 carotenoids, ß-carotene - orange colour) They Both are somers of CoH56. d)* The The lightly coloured componend is ß-carotence, which is eluked first, because dysopene has more double bonds so it Sticke to the column more Readily than ß-carotene * B-Carotene is less because it has less polar than lycopene conjugated fi Bonds. Bacarotene dycopene 9 11 Pi Bonds & 13 fé Bonds