Question

1.why is there a 90 degree configuration between the exciting beam and the optics of collecting fluoresecence in a real spectrofluorometer?

2. what sources of light can exit the sample at 90 degrees?

3. what do we most likely see when the detector is placed at 180 degrees ( as we do in an absorption spectrophotometer)

*please no cursive hand writing, please use normal block script / printed hand writing

Answer 1

Why is there a 90 degree configuration between the exciting beam and the optics of collecting fluorescence in a real spectrophotometer ? The detector is normally put at qo' Cviewing angle) which tries to prevent Incident light from polluting the detected fluorescent light. The hinorescence is normally collected at go with respect to the optical beam to maximize the efriciency of the emission collection and reduces the background . Acconding to updated chegs could only answen the 1st asked by you g. question