Question

Table 1: Red 40 Dye Solution [Red 40), M 0.1000 0.0800 0.0500 0.0200 0.0000 Absorbance, AU 0.348 0.295 0.147 0.075 0 Blank Analytical A, nm: Unknown No: Unknown Abs: Slope of B-L Plot: Int'pt of Plot: [Red 40) in unk: 501.9 B-74 0.792 3.529 -0.00347

PLEASE HELP! How would you solve for concentration (X) using the best fit line from the graph?

Answer 1

Answer to the problem: Beer- Lamberts Law A = E.col (1) A = Absorbance {=Molar Absortivity c = Concentration la Palth length Kinear equation for Calibration Curre- y = mx tb Gi) no y = Absorbance (A) mime = slope (Ert) b = Inlercept (y-axis) x = concentration (c) from equ? 6 and (i) A = E.Colt b ori Cuneous - unknown El from the graph y = 3.5294 2 to 0.0035 .. Slope = m = 8.1 = 3.529 Absorbance (A) of unknown = 0.792 A-b El UM Kyiv 0.792-(-0.003477 3.529 0,792 +0.00347 3.529 м Unknown - Cunknown = 0.226 M . [Red-40] in unknown = 0.226M Ans.