Question

Table 1: Red 40 Dye Solution А в [Red 40), M 0.1000 Т. 0.0800 0.0500 0.0200 0.0000 Absorbance, AU 0.348 0.295 0.147 0.075 0 D Blank Analytical , nm: Unknown No: Unknown Abs: Slope of B-L Plot: Int'pt of Plot: [Red 40] in unk: 501.9 B-74 0.792 3.529 -0.00347 PLEASE HELP! 1. Solve for concentration (x) using your best fit line from the graph (y=mx+b) 2. What is the concentration of your unknown solution in mM? 3. What is the molar absorptivity (e) of the drink mix? (In units of mM-1 cm-1)

Answer 1

I give you the detailed solution to this problem as follows, Hope it will help you.

Slope= 3.529412 Intercept= -0.00347 Absorbance 0 0.05 0.1 0.15 0.2 0.25 Concentration 0.3 0.35 0.4 AU The best fitted enoue is Shown above ③ from Lambert Beer's Law, A=Eodrę Absorbance concentration Ex Molar extingtion coefficient e => curette thickness Tant el so A vo e plot will give 1. Slope = 3.53 le til = 3.53 80 t = 3:53 | Cº - Mlant so € = 3.53 & 1000 mm-lcnt B = 3-53* 103 mm-lam' (Answer) € Unknown Absorbance =0.792 B (Giren) tol=3053 (Calculated - from Standard ceave) So Unknown concentration ez A -0.792 3.53 x 103 ( til = 2:24 *104 mm (Ans).