Question

This marks the distance the mobile phase traveled during elution Pheophytin Xanthophyll Chlorophylla Chlorophyll B Lutein Poorly resolved Band(s) This circle marks the position the mixture was deposited before elution. 74

please help answer measure each component retention factor and

Answer 1

What is retention factor, Rf ? The Rf is a quantitative indicatiort of how far a paticular compound travels in particular. solvent. The Rf value is a good indicator of wheather an unknown compound and a known compound are similar, if not identical. If the Rf value for the Unknown Compound is closed or the same as the Rf value for known compound then the two compounds are most likely similar or Identical. . The retention factor, Rf, is defined as - Distance travolled by the solute (A) Af a Distance travelled by the solvent (D2) Rf 2 D2 Di= distance travelled by colourband, measured from center of the band of colour to the point where the colont pour mixture was applied. total distance that solvent traveled Dq = Orlar ان الاندز، کار D ovom 32 d

solventfront ho r ita R * Solite foort i De boob el browotno rasonerie saabove We can on RL, using same concept & formula :- calculeito to Pheophytin inte Xanthophyll chlorophyll A a Dil chlorophyloco huteinkost le D3 D4 st selle vasst eristeita Distance toarolled by sdvent front (P) = 7.5cm Distance travelled by solute front (D2) Pheophylin)= 7.00 cm Distance travolled by solute foont Kanthophyll)(D3)-5-6 cm Distance travelled by solute front chlorophyll A (D4) -427 Distance travelled by soluto front chlorophyll B (DS) = 3.3cm Distance travelled by solute front hutein (16) = 1.5cm volled by sol font chlorophyll B (s) 1.5cm

Rr * Distance travelled by solute front Distance travelled By solveut toont Rf for Pheophytin - Poza 1s = 0.93 Rf for Xanthophyll = b = c = 0.74 Rt for chlorophylca) - B 9 = 0.62 Rf for chlorophyll B = D3 = 0.44 Rf for & hutein = D = Is i = 0.20 Table 1: Component T Rf Pheophylin 0.93 Xanthophyll | Chlorophylla 1. chlorophyll B I hatein Hn 0-74 0.62 0.44 0.20