Question

How do dipole moments predict the order of elution in reverse phase High-Performance Liquid Chromatography (HPLC)?

Answer 1

In reversed phase HPLC, stationary phase is non polar and mobile phase is polar. Most reversed phase separations are done in polar solvents.

The elution order of solutes in reversed phase HPLC is governed by polarity.For a normal phase separation, solutes of low polarity spends proportionally less time in polar stationary phase and are the first solutes to elute from the column. In reversed phase HPLC, the order of elution is opposite of that in normal phase separation, with more polar solute eluting first.Increasing the polarity of mobile phase leads to longer retention time.