There are only three factors in chromatography that affects the resolution. These are-
1) Retention 2) selectivity 3) Efficiency
The components of the HPLC are-
Pump , detector, column ( most important part of the chromatograph) , injector and data system
What factors can affect resolution in High Performance Liquid Chromatography? What are the components of a High Performance Liquid Chromatography instrument?
Please answer What are some factors that could affect the selectivity and the resolution in Gas Chromatography (GC) & Liquid Chromatography (LC), and How do we affect the separation in (GC)&(LC) ?
9. a) Draw a block diagram for a High Performance Liquid Chromatograph (HPLC), showing all the important components. Briefly describe the function of each part. b) List four types of stationary phase/separation methods that are available for HPLC and suggest suitable analytes or analyses for each type of stationary phase. c) List four detection options that exist for HPLC and discuss the good and bad points (if any) of each. d) Choose one aspects of chromatography (eg. resolution or sample...
How do dipole moments predict the order of elution in reverse phase High-Performance Liquid Chromatography (HPLC)?
Shown below is an injection valve used in high-performance liquid chromatography. Correctly label the valve that is in the load position, and the valve that is in the inject position. Syringe Syringe IE To waste To waste To column To column Inject position Solvent enters Solvent enters Load position
In this experiment, we are doing quantitative determination of Caffeine by reversed-phase high pressure liquid chromatography (HPLC). Elevated column temperature was employed in this method. What impact does increasing column temperature have on retention? On resolution?
In gas chromatography how does column length, temperature, and coating thickness affect the resolution?
What are the factors that affect spatial resolution and distortion? Explain each in detail.
Electrospray time‑of‑flight (TOF) mass spectrometry was used to analyze the eluate from a high‑performance liquid chromatography (HPLC) separation. The mass spectrum of one chromatographic peak, containing a protein of unknown molecular mass, displays MHnn+ peaks at m/z= 6636.908, 6194.524, 5807.480, 5465.932, 5162.360, and 4890.726. Find the average molecular mass (M) of the neutral protein and its standard deviation.
A forensic chemist runs High-Performance Liquid Chromatography-Mass Spectrometry (HPLC- MS) on seven hair samples from the same individual, suspect to contain traces of cocaine. The following concentrations were obtained in pg/mg: 1.8, 1.4, 0.75, 1.3, 1.6, 1.5, 1.7. Using the Grubb’s test, are there any outliers in the data? Show your work
Electrospray time‑of‑flight (TOF) mass spectrometry was used to analyze the eluate from a high‑performance liquid chromatography (HPLC) separation. The mass spectrum of one chromatographic peak, containing a protein of unknown molecular mass, displays MHn+n peaks at m/z= 6710.365, 6263.085, 5871.757, 5526.428, 5219.496, and 4944.856. Find the average molecular mass (M) of the neutral protein and its standard deviation.