In gas chromatography how does column length, temperature, and coating thickness affect the resolution?
In gas chromatography how does column length, temperature, and coating thickness affect the resolution?
Lab #2: Gas Chromatography Theory behind gas chromatography What do all types of chromatography share in common? Relationship between retention time and boiling point/vapor pressure How changing parameters affect the retention time Ramp rate Temperature Length of column Pressure Whether column is packed or unpacked
Please answer What are some factors that could affect the selectivity and the resolution in Gas Chromatography (GC) & Liquid Chromatography (LC), and How do we affect the separation in (GC)&(LC) ?
for gas chromotography, describe how and explain why column temperature affects resolution, retention and total analysis time
Chromatography column resolution Example: A column has an efficiency of 14,000 theoretical plates. It has a to value of 1.3 min. Two analytes have retention times of 10.4 and 12.2. Calculate their resolution factor
2. (5) How is column chromatography related to TLC? Gas chromatography? Be sure to discuss mobile phase, stationary phase, sample size, sample type in your answer. 3. (2) How is Rf in TLC related to elution order in column chromatography? What do you expect to come out first and last?
What factors can affect resolution in High Performance Liquid Chromatography? What are the components of a High Performance Liquid Chromatography instrument?
How can you improve the resolution between two closely spaced peaks in gas chromatography. Describe the process you would undertake to improve separation.
Calculate the vapor pressure of 3-pentanone (b.p. 102.0 °C) at the gas chromatography column temperature of 96.0"C using the form of the Clausius-Clapeyron equation shown below, P1 P. vap where R is the ideal gas constant, ???? is the enthalpy of vaporization, T1 and T2 are two different temperatures, and P1 and P2 are the vapor pressures at the respective temperatures. The enthalpy of vaporization can be estimated using Trouton's rule, ??'vap-(88 J mol-1 K-1), Tp. Number Torr Calculate the...
As you increase the gas chromatography column temperature, analytes’ ______________ increases and stationary phase solubility _______________. A) boiling point; increases B) vapor pressure; decreases C) vapor pressure; increases D) retention time; increases E) retention time; decreases
What are the advantages and disadvantages of thin-layer chromatography, adsorption column chromatography, and gas chromatography techniques? (advantages and disadvantages for each type)