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Chromatography column resolution Example: A column has an efficiency of 14,000 theoretical plates. It has a...
what will happen with the retention factor, separation factor, resolution and theoretical plates if a longer...
what will happen with the retention factor, separation factor, resolution and theoretical plates if a longer column is used.
To obtain a resolution of 1.50, how many theoretical plates must a packed column have for...
To obtain a resolution of 1.50, how many theoretical plates must a packed column have for two peaks which elute at 5.42 and 5.63 minutes, where tm = 1.00 min? With a resolution of 1.50, are these peaks considered to be resolved?
EXERCISE 6: Two chemical species, A and B are separated by isothermal gas chromatography, using a...
EXERCISE 6: Two chemical species, A and B are separated by isothermal gas chromatography, using a 2.00 m column having 5000 theoretical plates at a flow rate of 15.0 ml / min. The peak of unabsorbed air appears after 30 s; the peak of A appears after 5 min and that of B after 12 min (a) Calculate the dead volume VM of the column, and the retention volumes Va and VB. (b) Calculate the reduced volumes V’a and V'B...
3. a) b) c) How many theoretical plates produce a chromatography peak eluting at 4.78 min...
3. a) b) c) How many theoretical plates produce a chromatography peak eluting at 4.78 min with a full width at half max (FWHM) of 1.20 s? The length of the column is 30.0 m. What is the plate height in microns? What is the peak resolution for this peak and another peak eluted at 4.73 minutes that has a full peak width (measured at the base) of 2.14 seconds? Is this sufficient for quantitative analysis of those two components?...
L. Choose the correct answer: 1) Reversed phase liquid chromatography refers to (a) the use of...
L. Choose the correct answer: 1) Reversed phase liquid chromatography refers to (a) the use of non polar solvent as the eluent in HPLC. (b) the use of polar material as the stationary phase in a chromatographic column. the use of non-polar material as the stationary phase in a chromatographic column 2) A solute with a retention time of 325 seconds has a base width of 15 seconds. The column is 11.5 cm long. The c (2 points) olumn has...
Two compounds C and D are separated on a column 34 cm long . Given the...
Two compounds C and D are separated on a column 34 cm long . Given the chromatographic data below calculate The column resolution? , the number of theoretical plates ?the height of theoretical plate and what length of column would be required to give a resolution of 1.5? Compound C retention time 18.3 width at base of peak(min) = 2.18 Compound D retention time 17.7, width at base 1.23
With GC system efficiency of 50,000 theoretical plates and hold-up time of 20 sec. Calculate how...
With GC system efficiency of 50,000 theoretical plates and hold-up time of 20 sec. Calculate how many components could be separated between retention factors 2 and 8 assuming that the resolution between adjacent peaks is 1.5? (Show the derivation of solution formula).
Two isotopic compounds are produced in the synthesis of a new therapeutic drug, which must be...
Two isotopic compounds are produced in the synthesis of a new therapeutic drug, which must be separated by repeated chromatography cycles. In each cycle, the solution containing both compounds passes through a chromatography column of length ?=45.0 cm containing ? theoretical plates. The compounds have an adjusted relative retention, α, of 1.093 and retention factors of 6.09 and 6.66 , respectively. Calculate the number of theoretical plates, ? , in the column of length ? . The observed resolution is...
Chromatograms of compounds A and B were obtained at the same flowrate with two columns of...
Chromatograms of compounds A and B were obtained at the same
flowrate with two columns of equal length. The Value of tm is 1.3
min in both cases.
Chromatograms
of compounds A and B were obtained at the same flow rate with two
columns of equal length. The value of
tm is
1.3 min in both cases.
I Column 1 Column 2 12 10 Time What is the numcrical valac of the nenn factor of pak A what isthemererkal valse...
Consider a separation performed on a 45.0 mm long open tubular column with a 0.50 mm...
Consider a separation performed on a 45.0 mm long open tubular column with a 0.50 mm diameter, and a 2.0 um thick stationary phase. Compound A eluted at 12.41 min, compound B eluted at 13.34 min, and the unretained solvent eluted at 1.130 min. What are the adjusted retention times, t, and retention factors, k, for compounds A and B? Number Number min Number Number min rB What is the relative retention, a, for this separation? Number roll down to...
EXERCISE 6: Two chemical species, A and B are separated by isothermal gas chromatography, using a 2.00 m column having 5000 theoretical plates at a flow rate of 15.0 ml / min. The peak of unabsorbed air appears after 30 s; the peak of A appears after 5 min and that of B after 12 min (a) Calculate the dead volume VM of the column, and the retention volumes Va and VB. (b) Calculate the reduced volumes V’a and V'B...
3. a) b) c) How many theoretical plates produce a chromatography peak eluting at 4.78 min with a full width at half max (FWHM) of 1.20 s? The length of the column is 30.0 m. What is the plate height in microns? What is the peak resolution for this peak and another peak eluted at 4.73 minutes that has a full peak width (measured at the base) of 2.14 seconds? Is this sufficient for quantitative analysis of those two components?...
L. Choose the correct answer: 1) Reversed phase liquid chromatography refers to (a) the use of non polar solvent as the eluent in HPLC. (b) the use of polar material as the stationary phase in a chromatographic column. the use of non-polar material as the stationary phase in a chromatographic column 2) A solute with a retention time of 325 seconds has a base width of 15 seconds. The column is 11.5 cm long. The c (2 points) olumn has...
Chromatograms of compounds A and B were obtained at the same
flowrate with two columns of equal length. The Value of tm is 1.3
min in both cases.
Chromatograms
of compounds A and B were obtained at the same flow rate with two
columns of equal length. The value of
tm is
1.3 min in both cases.
I Column 1 Column 2 12 10 Time What is the numcrical valac of the nenn factor of pak A what isthemererkal valse...
Consider a separation performed on a 45.0 mm long open tubular column with a 0.50 mm diameter, and a 2.0 um thick stationary phase. Compound A eluted at 12.41 min, compound B eluted at 13.34 min, and the unretained solvent eluted at 1.130 min. What are the adjusted retention times, t, and retention factors, k, for compounds A and B? Number Number min Number Number min rB What is the relative retention, a, for this separation? Number roll down to...
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