what will happen with the retention factor, separation factor, resolution and theoretical plates if a longer column is used.
If a longer column is used than-
theoretical plates depends on the column length. It can be determined by the formula.
N= L/H
Where N is no. Of theoretical plates, L= column length, H= height of each plate.
Therefore if a longer column is used ,the number of theoretical plates increases.
Retention factor is actually , How much time a component spend in a stationary phase in comparison of non retained inert compound. Generally it doesn't depends on column length instead of retention Time.
Separation factors increases as the longer column is used in any chromatography technique
Resolution is actually, the ability to separate two peaks .it also increases as the longer column is used in chromatography.
what will happen with the retention factor, separation factor, resolution and theoretical plates if a longer...
Chromatography column resolution Example: A column has an efficiency of 14,000 theoretical plates. It has a to value of 1.3 min. Two analytes have retention times of 10.4 and 12.2. Calculate their resolution factor
A student researcher performed a chromatographic separation of caffeine and aspartame. The retention time for caffeine, is, was found to be 218.0 s with a baseline peak width, wc, of 14.4 s. The retention time for aspartame, 1., was 250.5 s with a baseline peak width, w of 21.1 s. The retention time for the unretained solvent methanol was 40.4 s. Calculate the average plate height, H, in micrometers for this separation, given that it was performed on a 27.7...
A student researcher performed a chromatographic separation of caffeine and aspartame. The retention time for caffeine, tc,was found to be 207.6 s with a baseline peak width, wc, of 16.2 s. The retention time for aspartame, ta, was 253.7 s with a baseline peak width, wa, of 18.7 s. The retention time for the unretained solvent methanol was 40.7 s. Calculate the average plate height, H, in micrometers for this separation, given that it was performed on a 27.3 cmlong...
Calculate: Retention factor (k), Separation factor (α), and Resolution (R) Gas Cromatograyphy of a mixture containing benzene, methyl toluene and chlorotoluene First Peak : 4.242 Second Peak : 5.645 Third Peak : 7.577
1. Listed below are retention times and peak widths for an HPLC chromatographic separation on a 10 cm C18 column a) Calculate the resolution between benzene and benzo[e]pyrene. Compound Unretained Benzene Benzo[e]pyrene Pyrene Coronene Time (min) Baseline Width (min) 0.9 10.9 13.3 16.7 26.1 0.40 0.40 0.45 0.50 b) Is this a good resolution, yes, no, why? c) Calculate the theoretical number of plates for benzo[elpyrene.
Consider a separation performed on a 45.0 mm long open tubular column with a 0.50 mm diameter, and a 2.0 um thick stationary phase. Compound A eluted at 12.41 min, compound B eluted at 13.34 min, and the unretained solvent eluted at 1.130 min. What are the adjusted retention times, t, and retention factors, k, for compounds A and B? Number Number min Number Number min rB What is the relative retention, a, for this separation? Number roll down to...
A student researcher performed a chromatographic separation of caffeine and aspartame. The retention time for caffeine, lc, was found to be 203.8 s with a baseline peak width, wc, of 13.8 s. The retention time for aspartame, ta, was 272.4 s with a baseline peak width, wa, of 21.5 s. The retention time for the unretained solvent methanol was 43.7 s Calculate the average plate height, H, in micrometers for this separation, given that it was performed on a 22.7...
To obtain a resolution of 1.50, how many theoretical plates must a packed column have for two peaks which elute at 5.42 and 5.63 minutes, where tm = 1.00 min? With a resolution of 1.50, are these peaks considered to be resolved?
consider the peaks for trans-chlordane and for cis-chlordane where its retention time is 458.5 seconds and 475.0 seconds respectively. The elution time for unretained solute is 25 seconds. The width base for t-chlordane is 4 seconds and c-chlordane is 4.5 seconds. The open tubular column is 12.5m in length and 0.32 in diameter. Find the adjusted retention time, retention factor, number of theoretical plates and plate height for t-chlordane and c-chlordane peaks. in addition calculate the selectivity factor and resolution...
The table contains data for a liquid chromatography separation of four compounds, A, B, C, and D, on a 40.0 cm column Retention Compound time (min) Width at the base (min) A 5.17 0.436 B 1.02 13.0 15.0 C 1.10 23.4 1.91 Calculate the number of theoretical plates, N, for each peak. NA NB Nc ND = What is the average number of theoretical plates, Navg, for Navg the column? What is the plate height, H, for the column? Н-...