what will happen with the retention factor, separation factor, resolution and theoretical plates if a longer...
what will happen with the retention factor, separation factor, resolution and theoretical plates if a longer column is used.
Homework Answers
If a longer column is used than-
theoretical plates depends on the column length. It can be determined by the formula.
N= L/H
Where N is no. Of theoretical plates, L= column length, H= height of each plate.
Therefore if a longer column is used ,the number of theoretical plates increases.
Retention factor is actually , How much time a component spend in a stationary phase in comparison of non retained inert compound. Generally it doesn't depends on column length instead of retention Time.
Separation factors increases as the longer column is used in any chromatography technique
Resolution is actually, the ability to separate two peaks .it also increases as the longer column is used in chromatography.
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what will happen with the retention factor, separation factor,
resolution and theoretical plates if a longer...
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Chromatography column resolution Example: A column has an efficiency of 14,000 theoretical plates. It has a to value of 1.3 min. Two analytes have retention times of 10.4 and 12.2. Calculate their resolution factor
A student researcher performed a chromatographic separation of caffeine and aspartame. The retention time for caffeine,...
A student researcher performed a chromatographic separation of caffeine and aspartame. The retention time for caffeine, is, was found to be 218.0 s with a baseline peak width, wc, of 14.4 s. The retention time for aspartame, 1., was 250.5 s with a baseline peak width, w of 21.1 s. The retention time for the unretained solvent methanol was 40.4 s. Calculate the average plate height, H, in micrometers for this separation, given that it was performed on a 27.7...
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A student researcher performed a chromatographic separation of caffeine and aspartame. The retention time for caffeine, lc, was found to be 203.8 s with a baseline peak width, wc, of 13.8 s. The retention time for aspartame, ta, was 272.4 s with a baseline peak width, wa, of 21.5 s. The retention time for the unretained solvent methanol was 43.7 s Calculate the average plate height, H, in micrometers for this separation, given that it was performed on a 22.7...
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